قارچ شناسی پزشکی
دانشکده علوم پزشکی- دانشگاه تربیت مدرس، تهران، جمهوری اسلامی ایران
قارچ شناسی پزشکی
دانشکده علوم پزشکی- دانشگاه تربیت مدرس، تهران، جمهوری اسلامی ایران
دانشگاه علوم پزشکی ایران، تهران، جمهوری اسلامی ایران
محمد حسین یادگاری متولد سال1337 در شهر تهران و دوران تحصیلات ابتدایی ومتوسطه را در این شهر گذرانده است در سال 70 با مرتبه مربی به استخدام دانشکده علوم پزشکی دانشگاه تربیت مدرس درامد تا کنون نیز بعداز فارغ التحصیلی در مقطع دکتری در سال 80 با مرتبه دانشیاری عضو هیات علمی گروه قارچ شناسی پزشکی ان دانشکده میباشددر چند دوره مدیر گروه بوده در سالهای تیر ماه89لغایت مهر 92 ریاست جهاد دانشگاهی کشوررا بعهده داشته است از سال 91 تا کنون عضو بورد تخصصی رشته خود میباشد
BackgroundBiofilm formation by Candida species is an influential virulence factor in candidemia pathogenesis. We investigated the relationship between biofilm formation of Candida tropicalis isolates with the clinical characteristics and mortality outcomes in patients with candidemia.Materials and methodsThirty-nine C. tropicalis isolates were recovered from patients with candidemia admitted to two university hospitals in Tehran, Iran. Biofilm mass and metabolic activity of C. tropicalis biofilms were assessed in vitro with two colorimetric methods. The sessile minimum inhibitory concentrations (SMICs) were evaluated in vitro by treating preformed biofilms with diluted concentrations of azoles according to CLSI-M27 A3/S4 protocol, followed
BackgroundVirulence factors intensify the pathogenicity of Candida species in candidemia. Limited knowledge exists regarding the azole-resistant mechanism and virulence factors of Candida tropicalis. Consequently, we aimed to evaluate the virulence factors and the molecular mechanisms of azole resistance among C. tropicalis isolated from bloodstream infection.
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Background and Objective: Nowadays opportunistic fungi especially C. albicans are the most common cause of life-threating infections in immunodeficiency patients. Increasing Azole-resistant strains of C. albicans are a main problem in human immunodeficiency virus-infected patients. The aim of this study was the evaluation of ERG11 gene in C. albicans Azole resistant strains?that were isolated from AIDS patients with oropharyngeal candidiasis by RT-PCR method. Materials and Methods: The present experimental study was conducted at Tarbiat Modares University during the years 2009-2011. C.albicans isolates from?HIV-infected patients were identified by standard procedures including germ tube formation,?clamydoconidia and color of colonies on CHR
ObjectiveTrichophyton verrucosum is a slow growing dermatophyte responsible for a number of skin diseases such as ringworm, and is characterized by patches of hair loss and thick crusts on the host skin in domestic animals. In this study, we examined the immunomodulatory effects of crude extract of Trichophyton verrucosum (TV)cytoplasm in a mouse model.MethodsThe TV variate was cultured on Sabouraud dextrose agar and the mycelium was grinded by mechanical force. The purified protein was obtained from crude extract of the fungus, and protein concentration was measured by BradFord assay. Six to eight week-female BALB/c mice were divided into three groups: test group, receiving cytoplasmic crude extract plus defibrinated sheep blood; control g
Secreted aspartyl proteinases (Saps), are gene products that have been shown to directly contribute to Candida albicans pathogenicity. Despite the clear difficulties of systemic C. albicans infections control, Antimicrobial peptides (AMPs) are regarded as one of the most promising alternatives in this regard. Recently, drug-loaded electrospun nanofibers have attracted a great deal of attention. In this study we have established the nanofibrous scaffold of new synthetic peptide/Poly (Vinyl Alcohol)/Poly l-Lactic Acid on expression of Secretory aspartyl proteinases 4 to 6 genes of C. albicans in comparison with free peptide. We designed new synthetic Antimicrobial peptide (AMPs) used bioinformatics tools to predict structure and stabilities.
ObjectiveThe effect of candidemia on immunologic parameters in breast tumor bearing patients is not well studied. Here, we hypothesised that candidemia in the tumor background may change the outcome of immunologic parameters and tumor condition.MethodMice were divided into four groups, including normal, tumor, Candida infected (only Candidiasis) and tumor/Candidiasis groups. Tumor changes were recorded daily after tumor transplantation and induction of candidemia. Splenocytes of mice were harvested, cultured, and stimulated with PHA; afterwards, IL-4, IL-10, IFN-γ, TNF-α and TGF-β cytokines were assessed using ELISA kits. We also evaluated the population of CD4+CD25+Foxp3+ regulatory T cells in the tumor infiltrated and splenocytes.Resul
An experiment was conducted to study the effect of savory (Satureja khuzistanica) extract on the performance, organ weight, immune response and hepatic enzymes in broiler chickens. 320 day-old Ross chickens were assigned to four distinct treatments in a completely randomized design. Each treatment was administered to four replicates of twenty birds. The variables were heat stress (34 ? 2 ˚C for 8 hours) and savory extract (0.4 ml/L) in drinking water. Feed intake (FI), weight gain (WG) and feed conversion ratio (FCR) were measured in successive weeks of the trial. The relative weights of different organs (dressing, breast, thigh, liver, heart, spleen and bursa of Fabricius) determined at 42 days. The serum glucose and blood plasma content
To investigate the efficiency of savory essential oil on performance, organs relative weight, some blood parameters, immune response and hepatic enzymes of broiler chickens, a total 320 male Ross 308 chicks were allocated to four treatments and four replicates of twenty birds as a completely randomized design from. The vari different levels savory oil. The body weight gain (BWG), feed intake (FI) and feed conversion ratio (FCR) was recorded at continues weeks of the experiment. Relative weight of visceral organs was determined and blood serum glucose and activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) were measured after blood sampling at 42 days old and plasma IgG immunoglobuli
Background: The importance of Candida albicans as the most common cause of fungal infections in humans is undeniable. Genotyping methods have been developed as useful tools to differentiate between fungal strains isolated from various infections. Several molecular typing methods have been described for C. albicans, and fragment length analysis of microsatellites called microsatellite fragment length polymorphism (MLP) is one of the most accurate genotyping methods.Objectives: The present study aimed at evaluating the genetic diversity and genetic relationships among C. albicans isolates recovered from HIV-positive patients with oral candidiasis in Iran using MLP.Methods: We analyzed 30 isolates of C. albicans obtained from HIV-positive pati
Background: Candida albicans is the most common pathogen in patients with vulvovaginitis. Secretory aspartyl proteinases (SAPs) are potential factors in the virulence of C. albicans. Antimicrobial peptides (AMPs) are recognized as a promising antimicrobial group of drugs with a membrane degradation mechanism. Occurrence of resistance to these drugs is quite rare. The impact of rapid membrane degradation on AMPs is relative to the prevention or delay in drug resistance mechanisms in different microbes.Objectives: The purpose of this study was to compare the inhibitory effects of the designed NK95 peptide and caspofungin on expression of SAP4-SAP6 genes from clinical vaginal isolates of C. albicans.Methods: In this experimental study, samples
Background: Candida albicans is the most common pathogen in patients with vulvovaginitis. Secretory aspartyl proteinases (SAPs) are potential factors in the virulence of C. albicans. Antimicrobial peptides (AMPs) are recognized as a promising antimicrobial group of drugs with a membrane degradation mechanism. Occurrence of resistance to these drugs is quite rare. The impact of rapid membrane degradation on AMPs is relative to the prevention or delay in drug resistance mechanisms in different microbes. Objectives: The purpose of this research was to explore the inhibitory effects of the designed NK95 peptide on SAP4-SAP6 gene expression from clinical vaginal isolates of C. albicans in comparison with caspofungin. Methods: In this experimenta
Materials and Methods:Vaginal samples of 68 women with suspected vaginitis were obtained and cultured. Canida albicans species were identified using phenotypic and genotyping methods. Spectrophotometry was used to investigate the presence of SAP protein in the vaginal samples, and SDS-PAGE was used to confirm its protein composition. Real-time PCR was performed to ascertain the effects of subinhibitory concentrations of Echinophora platyloba extract, carvacrol and caspofungin on the expression of SAP1-3 genes before and after treatment.Results:C. albicans was found as the abundant species (59.6%), and different amounts of SAP were present in all vaginal samples, which were higher than Candida krusei strain. The protein composition of SAP in
Objective: Candida albicans (C. albicans) is an opportunistic yeast that can lead to pathogenesis in immunocompromised individuals and under suitable conditions. Medicinal plants ingredients such as camphor can reduce the expressions of genes involved in virulence of the fungi through their antifungal properties. The products of INT1 and EFG1 are implicated in inducing filamentous growth and adhesion of C. albicans to the host tissues. Both of these characteristics are very important in its virulence. The present study focuses on the evaluation of the effects of camphor on INT1 and EFG1 expressions at three time points of treatment (24, 48, and 72 hours) via real-time PCR. Methods: We prepared serial dilutions of camphor (1, 2, 4, 8, 16, 32
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