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  • دکتری (1383)

    متخصص در ژنتیک پزشکی و فوق دکتری در انکوژنتیک

    مونپولیه، فرانسه

  • اپی ژنتیک در بیماریهای ژنتیک / سرطان و سلولهای بنیادی
  • ناباروری/ PGD/درمانهای جدید

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    A Panel of Plasma miRNAs 199b-3p, 224-5p and Let-7d-3p as Non-Invasive Diagnostic Biomarkers for Endometriosis

    Narges Zafari, Azam Manshadi Tarafdari, Pantea Izadi, Mehrdad Noruzinia, Mir Saead Yekaninejad, Afshin Bahramy, Ali Mohebalian
    Journal PapersReproductive Sciences , 2021 January 4, {Pages 09-Jan }

    Abstract

    The objective of this study was to investigate whether the combination of miR-224-5p, miR-199-3p, and let-7d-3p is a suitable diagnostic panel for endometriosis. Twenty-five women with endometriosis (case) and twenty-five women without any sign of endometriosis (controls) were included. Peripheral blood specimens were collected from all these women who were a proper candidate for laparoscopy before surgery. Total RNA was isolated to synthesize complementary DNA. Expression of miR-199b-3p, miR-224-5p, and let-7d-3p was analyzed by RT-qPCR. To estimate the performance of the identified miRNAs for endometriosis diagnosis, we performed ROC curves analysis. There was an upregulation of miRNAs 199b-3p (P value < 0.001) and 224-5p (P

    Alterations of miR-16, miR-let-7a and their target genes expression in human blastocysts following vitrification and re-vitrification

    M Daneshvar, M Movahedin, M Salehi, M Noruzinia
    Journal Papers , , {Pages }

    Abstract

    The Role of miRNAs 340-5p, 92a-3p, and 381-3p in Patients with Endometriosis: A Plasma and Mesenchymal Stem-Like Cell Study

    A Bahramy, N Zafari, P Izadi, F Soleymani, S Kavousi, M Noruzinia
    Journal Papers , , {Pages }

    Abstract

    Dysregulation of Angiogenesis and Inflammatory Genes in Endometrial Mesenchymal Stem Cells and Their Contribution to Endometriosis

    S Heydari, L Kashani, M Noruzinia
    Journal Papers , , {Pages }

    Abstract

    Young Breast Cancer: Novel Gene Methylation in WBC

    M Noruzinia, J Tavakkoly-Bazzaz, M Ahmadvand, F Azimi, ...
    Journal Papers , , {Pages }

    Abstract

    The Epigenetic Assessment of Human Spermatogenic Cells Derived from Obstructive Azoospermic Patients in Different Culture Systems

    M Zahiri, M Movahedin, SJ Mowla, M Noruzinia, M Koruji
    Journal Papers , , {Pages }

    Abstract

    Indirect Tumor Inhibitory Effects of MicroRNA-124 through Targeting EZH2 in The Multiple Myeloma Cell Line

    Javid Sabour Takanlu, Arad Aghaie Fard, Saeed Mohammdi, Mohsen Nikbakht, Negin Afrang, Rezvan Tavakoli, Nooshin Tasharrofi, Fatemeh Kouhkan, Farnaz Razmkhah, Sorayya Ghasemi, Masoud Soleimani, Sedigheh Amini Kafi-abad, Arezou Mehrabi, Nafiseh Baheiraei, M
    Journal PapersCell Journal (Yakhteh) , Volume 22 , Issue 1, 2020 March 1, {Pages }

    Abstract

    Objective: Multiple myeloma (MM) is an incurable plasma cell malignancy. Several genetic and epigenetic changes affect numerous critical genes expression status in this disorder. CDKN2A gene is expressed at low level in almost all cases with MM disease. The mechanism of this gene down-regulation has remained controversial. In the present study, we targeted EZH2 by microRNA-124 (miR-124) in L-363 cells and assessed following possible impact on CDKN2A gene expression and phenotypic changes.Materials and Methods: In this experimental study, growth inhibitory effects of miR-124 were measured by MTT assay in L-363 cell line. Likewise, cell cycle assay was measured by flowcytometery. The expression levels of EZH2 and CDKN2A were evaluated by real

    Identification of candidate microRNA markers of endometriosis with the use of next-generation sequencing and quantitative real-time polymerase chain reaction

    Elahe Papari, Mehrdad Noruzinia, Ladan Kashani, Warren G Foster
    Journal PapersFertility and Sterility , Volume 113 , Issue 6, 2020 June 1, {Pages 1232-1241 }

    Abstract

    ObjectiveTo identify novel candidate diagnostic microRNA (miRNA) markers of endometriosis by means of an unbiased search with confirmation by means of targeted polymerase chain reaction (PCR).DesignRetrospective cohort.SettingUniversity teaching hospitals.Patient(s)Women with endometriosis and control women, confirmed with the use of laparoscopy.Interventions(s)Diagnostic laparoscopy and blood sample.Main Outcome Measure(s)Next-generation sequencing (NGS) and quantitative real-time PCR (qRT-PCR).Result(s)Candidate miRNAs differentially expressed in women with endometriosis compared with control women were identified by means of NGS and selected for qRT-PCR. Plasma samples from another cohort of women with surgically confirmed endometriosis

    Gene expression analysis signifies the association of inflammatory proteins with the development of endometriosis

    Shermineh Heydari, Ladan Kashani, Mehrdad Noruzinia
    Journal PapersGene Reports , 2020 May 25, {Pages 100716 }

    Abstract

    Endometriosis is primarily defined by the presence of endometrial glands and stroma outside of the uterine cavity. The diagnosis and management of the disease are challenging because of the unclear pathogenesis and heterogeneity of clinical symptoms.The main focus of the current study is to analyze the gene expression profiles of individuals with endometriosis and to clarify key candidate genes and pathways involved in the disease pathogenesis using the integrated bioinformatics analysis. The following mRNA expression profile datasets, namely GSE23339 and GSE25628, were obtained from the Gene Expression Omnibus database and differentially expressed genes (DEGs) with the following criteria p < 0.05 and [logFC] > 1 were identified. To

    Deregulation of Stemness-Related Genes in Endometriotic Mesenchymal Stem Cells: Further Evidence for Self-Renewal/Differentiation Imbalance

    Parisa Mashayekhi, Mehrdad Noruzinia, Sepideh Khodaverdi
    Journal PapersIranian Biomedical Journal , Volume 24 , Issue 5, 2020 September , {Pages 328 }

    Abstract

    Background:Any irregularities in self-renewal/differentiation balance in endometriotic MSCs can change their fate and function, resulting in endometriosis development. This study aimed to evaluate the expression of OCT4 transcripts (OCT4A, OCT4B, and OCT4B1), SOX2, and NANOG in endometriotic MSCs to show their aberrant expression and to support self-renewal/differentiation imbalance in these cells.Methods:MSCs were isolated from three endometriotic and three normal endometrium samples and characterized and analyzed for the expressions of OCT4A, OCT4B, OCT4B1, SOX2, and NANOG using the qRT-PCR.Results:The expressions of OCT4 transcripts and NANOG increased significantly in endometriotic MSCs, whereas SOX2 expression did not show any signific

    Differential expression of microRNAs associated with apoptosis and implantation in human in vitro-produced blastocysts following re-vitrification

    M Daneshvar, M Movahedin, M Salehi, M Noruzinia
    Conference PapersHUMAN REPRODUCTION , Volume 35 , 2020 July 1, {Pages I232-I232 }

    Abstract

    The Epigenetic Assessment and Proliferation of Human Spermatogenic Cells Derive from Obstructive Azoospermic Patients in Different Culture Systems

    Maria Zahiri, Seyed Javad Mowla, Mehrdad Noruzinia, Morteza Koruji, Mohammad Reza Nowroozi, Zahra Bashiri
    Journal PapersUrology Journal , 2020 November 23, {Pages 6092-6092 }

    Abstract

    Purpose: Generating functional gametes for patients with male infertility is of great interest. We investigated different cultural systems for proliferation of SSCs derived from obstructive azoospermic patients.Materials and Methods: Testicular cells were obtained from men with obstructive azoospermia. after enzymatic digestion process, cells assigned to various groups: culture of SSCs in the dish without cover (control group), co-culture of SSCs with infertile Sertoli cells (I), co-culture of SSCs with fertile Sertoli cells (II), culture of SSCs on nanofiber (covered with laminin)(III), culture of testicular cell suspension (IV). Then cells were cultured and evaluated colony formation, gene-specific methylation by MSP, quantitative genes e

    Endometriotic Mesenchymal Stem Cells Epigenetic Pathogenesis: Deregulation of miR-200b, miR-145, and let7b in A Functional Imbalanced Epigenetic Disease.

    Parisa Mashayekhi, Mehrdad Noruzinia, Sirous Zeinali, Sepideh Khodaverdi
    Journal PapersCell Journal (Yakhteh) , Volume 12 , Issue 2, 2019 July 1, {Pages }

    Abstract

    Objective: Stem cell issue is a strong theory in endometriosis pathogenesis. It seems that endometriotic mesenchymal stem cells (MSCs) show different characteristics compared to the normal MSCs. Determined high proliferation and low differentiation/decidualization potential of endometriotic MSCs could be accompanied by their microRNAs deregulation influencing their fate and function. In this study for the first time, we evaluated the expression of miR-200b, miR-145, and let-7b in endometriotic compared to non-endometriotic MSCs. These microRNAs are involved in biological pathways related to proliferation and differentiation of stem cells. Their aberrant expressions can disturb the proliferation/differentiation balance in stem cells, alterin

    Conditioned stem cell-derived extract

    Journal Papers , 2019 May 23, {Pages }

    Abstract

    A method for modifying gene expression of diseased cells in a patient including preparing an extract of diseased cells, forming a plurality of conditioned stem cells by treating a plurality of normal stem cells with a solution of the extract of the diseased cells with a volume ratio between 10− 15 volume/volume (v/v) and 10− 3 v/v (volume of the extract of the diseased cells/volume of a culture medium), forming a conditioned stem cell-derived extract, and forming a plurality of healthy cells by treating the diseased cells with the conditioned stem cell-derived extract.

    Optimization of Antioxidant Peptides Production from the Mantle of Cuttlefish (Sepia pharaonis) Using RSM and Fractionation

    Ali Hamzeh, Masoud Rezaei, Saber Khodabandeh, Ali Motamedzadegan, Mehrdad Noruzinia, Joe Mac Regenstein
    Journal PapersJournal of Aquatic Food Product Technology , Volume 28 , Issue 4, 2019 April 21, {Pages 392-401 }

    Abstract

    Persian Gulf cuttlefish mantles were hydrolyzed (CPH) using alcalase, and the optimal hydrolysis parameters were obtained for the highest degree of hydrolysis (DH) and strongest antioxidant (based on their ability to quench?1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals) activity using response surface methodology (RSM). The predicted optimal parameters of DH and quenching DPPH radicals was: pH of 7.88, 50.2?C, 150?min, and enzyme to substrate ratio of 1.5%. The reducing power (RP) and ability of optimized peptides to quench ABTS radicals in a gastro-intestinal track model system increased during the intestinal stage, while scavenging ability against DPPH radicals dropped (P

    Possible dual contribution of a novel GUCY2D mutation in the development of retinal degeneration in a consanguineous population

    Ahmad Reza Salehi Chaleshtori, Masoud Garshasbi, Ali Salehi, Mehrdad Noruzinia
    Journal PapersEuropean journal of medical genetics , 2019 August 27, {Pages 103750 }

    Abstract

    Molecular characterization of novel mutations in Leber Congenital Amaurosis (LCA) disease improves the disease diagnosis and contributes to the development of preventive and therapeutic approaches. We studied an isolated inbred population in Iran with a high prevalence of retinal degeneration with clinical variability. The clinical examinations were performed on eight patients belonging to three consanguineous families. The identical-by-descent (IBD) mapping technique was employed to identify the shared loci in patients. Subsequently, Sanger sequencing of the GUCY2D gene, in silico analysis, as well as segregation study were conducted. The whole-exome sequencing method was applied for negative cases of GUCY2D mutation, followed by segregati

    The identification and stereochemistry analysis of a novel mutation p.(D367Tfs* 61) in the CYP1B1 gene: A case report

    Ahmad Reza Salehi Chaleshtori, Masoud Garshasbi, Ali Salehi, Mehrdad Noruzinia
    Journal PapersJournal of Current Ophthalmology , 2019 November 15, {Pages }

    Abstract

    PurposeTo investigate the presence of a probable genetic defect(s) that may cause primary congenital glaucoma (PCG) in a seven-year-old female patient.MethodsA seven-year-old female patient and her family received genetic counseling and underwent full clinical examinations by an expert ophthalmologist. The patient's genomic DNA was subjected to the targeted gene capture and next-generation sequencing (NGS) along with Sanger sequencing method. The 3D structure prediction and stereochemistry analysis were performed for both mutant and wild-type forms of the CYP1B1 protein.ResultsThe clinical examinations indicated that the diagnosis of PCG was correctly made. We identified a novel homozygous deletion in which a “C” nucleotide was deleted

    Synergistic effect of simvastatin and romidepsin on gamma-globin gene induction

    Habibi Hussain, Atashi Amir, Abroun Saeid, Noruzinia Mehrdad
    Journal Papers , 2019 January , {Pages 576-583 }

    Abstract

    Objective:Hemoglobinopathies such as beta-thalassemia and sickle cell disease [SCD] are inherited disorders that are caused by mutations in beta-globin chain. Gamma-globin gene reactivation can ameliorate clinical manifestations of betathalassemia and SCD. Drugs that induce fetal hemoglobin [HbF] can be promising tools for treatment of beta-thalassemia and SCD patients. Recently, it has been shown that Simvastatin [SIM] and Romidepsin [ROM] induce HbF. SIM is a BCL11a inhibitor and ROM is a HDAC inhibitor and both of these drugs are Food and Drug Administration [FDA]-approved for hypercholesterolemia and cutaneous T-cell lymphoma respectively. Our aim was to evaluate the synergistic effects of these drugs in inducing HbF

    Endometriotic mesenchymal stem cells epigenetic pathogenesis: deregulation of miR-200b, miR-145, and let7b in a functional imbalanced epigenetic disease

    P Mashayekhi, M Noruzinia, S Zeinali, S Khodaverdi
    Journal Papers , , {Pages }

    Abstract

    A novel 8-bp duplication in ADAT3 causes mild intellectual disability

    Ahmad Reza Salehi Chaleshtori, Noriko Miyake, Mohammad Ahmadvand, Oranous Bashti, Naomichi Matsumoto, Mehrdad Noruzinia
    Journal PapersHuman genome variation , Volume 5 , Issue 1, 2018 May 21, {Pages 7 }

    Abstract

    Inosine is a base located at wobble position 34 of the tRNA anticodon stem–loop, enabling the recognition of more than one codon in the translation process. A heterodimer consists of ADAT3 and ADAT2 and is involved in the adenosine-to-inosine conversion in tRNA. Here, we report the second novel ADAT3 mutation in a patient with microcephaly, intellectual disability, and hyperactivity. These findings constitute a second mutation and expand the clinical spectrum of extremely rare ADAT3 mutations.

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    • دكتري
      روش هاي مولكولي و سيتو ژنتيك در تشخيص بيماري هاي خوني ( واحد)
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      نشانه شناسي بيماري ها ( واحد)
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      ژنتيك سرطان ( واحد)
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      ژنتيك پزشكي پيشرفته ( واحد)
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      نراقي, مونامعصومه
      تاثير سكرتوم سلول هاي بنيادي مزانشيمي سالم بر روي سلولهاي بنيادي مزانشيمي مالتيپل ميلوما از نظر تغيير در متيلاسيون ژنهاي منتخب و آپوپتوزيس ناشيBortezomib
    • مدیر گروه ژنتیک پزشکی
    • مدیر گروه آموزشی ژنتیک پزشکی
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