En
  • دکتری (1380)

    بیو شیمی

    دانشگاه تهران، ایران

  • کارشناسی‌ارشد (1374)

    بیو شیمی

    دانشگاه تهران، ایران

  • مطالعات پروتئومیکس در بیماری ها و فرآیندهای زیستی
  • تثبیت آنزیم ‌ها بر نانو ذرات و استفاده از آن در زیست حسگر بر اساس الکتروشیمی و SPR
  • تکنولوژی آنزیم و رابطه فعالیت- پایداری در آنزیم ها
  • آنزیم هاو پروتئین های دارویی: ساخت و انتقال در نانو ذرات
  • آنزیم

    زمینه های پژوهشی:

    کارشناس مسئول: زهره جعفری کلهر

    تلفن:

    مکان: دانشکده علوم زیستی طبقه اول-آزمایشگاه 4107

داده ای یافت نشد

ارتباط

رزومه

Cancer‐associated‐platelet‐inspired nanomedicines for cancer therapy

Marzieh Geranpayehvaghei, Bahareh Dabirmanesh, Mohammad Khaledi, Mona Atabakhshi‐Kashi, Chao Gao, Mohammad Taleb, Yinlong Zhang, Khosro Khajeh, Guangjun Nie
Journal Papers , 2021 February 3, {Pages e1702 }

Abstract

Platelets, with hemostasis and thrombosis activities, are one of the key components in the blood circulation. As a guard, they rapidly respond to any abnormal blood vessel injury signal and release their granules' contents, which induce their adhesion and aggregation on wound site for hemostasis. Recently, increasing evidence has indicated that platelets are critically involved in the growth and metastasis of cancer cells by releasing a variety of cytokines and chemokines to stimulate cancer cell proliferation and various angiogenic regulators to accelerate tumor angiogenesis. Platelets also secrete active transforming growth factor beta (TGF‐β) to promote the epithelial–mesenchymal transition of cancer cells and their extravasation f

Monophosphoramide derivatives: synthesis and crystal structure, theoretical and experimental studies of their biological effects

Khodayar Gholivand, Zahra Roshanian, Maryam Rahimzadeh Dashtaki, Zahra Hosseini, Ali Asghar Ebrahimi Valmoozi, Mahboobeh Sharifi, Fahimeh Mohammadpanah, Maryam Rajabi, Mohammad Ghadamyari, Sedigheh Farshadian, Reza Hasan Sajedi, Khosro Khajeh, Neda Akbari
Journal PapersMolecular Diversity , 2021 January 2, {Pages 16-Jan }

Abstract

Synthesizing new chemical compounds and studying their biological applications have been important issues in scientific research. In this investigation, we synthesized and characterized ten new N-acetyl phosphoramidate compounds and explored the crystal structure of three others. Furthermore, not only were some kinetic inhibition parameters measured, like IC 50, K i, k p, K D for 7 compounds on human acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), but also their hydrophobic parameter was determined by shake-flask technique. All compounds (number 1–10) were investigated for anti-bacterial activity against three Gram-positive and three Gram-negative bacteria, while chloramphenicol was used as a standard antibiotic. In order to

Urea titration of a lipase from Pseudomonas sp. reveals four different conformational states, with a stable partially folded state explaining its high aggregation propensity

Minoo Qafary, Khosro Khajeh, Matteo Ramazzotti, Ali Akbar Moosavi-Movahedi, Fabrizio Chiti
Journal PapersInternational Journal of Biological Macromolecules , Volume 174 , 2021 March 31, {Pages 32-41 }

Abstract

The conversion of soluble proteins into amyloid fibrils has importance in protein chemistry, biology, biotechnology and medicine. A novel lipase from Pseudomonas sp. was previously shown to have an extremely high aggregation propensity. It was therefore herein studied to elucidate the physicochemical and structural determinants of this extreme behaviour. Amyloid-like structures were found to form in samples up to 2.5–3.0?M using Thioflavin T fluorescence and Congo red binding assays. However, dynamic light scattering (DLS), static light scattering and turbidimetry revealed the existence of aggregates up to 4.0?M urea, without amyloid-like structure. Two monomeric conformational states were detected with intrinsic fluorescence, 8-anilinona

An experimental investigation on the influence of various buffer concentrations, osmolytes and gold nanoparticles on lysozyme: Spectroscopic and calorimetric study

Soraya Mohammadi, Khosro Khajeh, Majid Taghdir, Bijan Ranjbar
Journal PapersInternational Journal of Biological Macromolecules , Volume 172 , 2021 March 1, {Pages 162-169 }

Abstract

Considering importance and several industrial applications of lysozyme, including natural antibiotic and preservative, identifier for the diagnosis of diseases, and extraction purposes, its reversibility and stability studies can be very important. In this paper, the role that buffer and osmolytes concentrations play on the thermodynamic stability of lysozyme denaturation process, that is a new simple and inexpensive method, was evaluated by Nano-DSC III, far- and near-UV CD and fluorescence techniques. In thermal denaturation study, RI and ΔG of protein increased from 25.62% to 58.82% and 48.87 to 63.63?kJ?mol−1 with the increment of buffer and osmolytes concentrations, respectively. These changes showed a significant increase of 129.59

Screening and Identification of Herbal Urease Inhibitors Using Surface Plasmon Resonance Biosensor

Mahmood Biglar, Hafezeh Salehabadi, Safoura Jabbari, Bahareh Dabirmanesh, Khosro Khajeh, Faraz Mojab, Massoud Amanlou*
Journal PapersResearch Journal of Pharmacognosy , Volume 8 , Issue 2, 2021 April 1, {Pages 51-62 }

Abstract

Background and objectives: Urease, that catalyzes the hydrolysis of urea, has received substantial attention for its impact on living organisms’ health and human life quality. Urease inhibitors play important role in management of different diseases including gastritis and other gastrointestinal disorders. In the present study, a new surface plasmon resonance-based biosensor was designed to discover new urease inhibitors. Methods: The biosensor surface was prepared by the covalent immobilization of urease on carboxymethyldextran hydrogel (CMD 500D) via its primary amine groups. Results: The biosensor combined with an orthogonal enzyme inhibition assay was utilized for screening of 40 traditional medicinal plant extracts against Jack-bean

Proteomic profiling of the rat hippocampus from the kindling and pilocarpine models of epilepsy: potential targets in calcium regulatory network

Leila Sadeghi, Albert Anatolyevich Rizvanov, Bahareh Dabirmanesh, Ilnur Ildusovich Salafutdinov, Mohammad Sayyah, Amir Shojaei, Javad Zahiri, Javad Mirnajafi-Zadeh, Babak Khorsand, Khosro Khajeh, Yaghoub Fathollahi
Journal PapersScientific reports , Volume 11 , Issue 1, 2021 April 15, {Pages 12-Jan }

Abstract

Herein proteomic profiling of the rat hippocampus from the kindling and pilocarpine models of epilepsy was performed to achieve new potential targets for treating epileptic seizures. A total of 144 differently expressed proteins in both left and right hippocampi by two-dimensional electrophoresis coupled to matrix-assisted laser desorption-mass spectrometry were identified across the rat models of epilepsy. Based on network analysis, the majority of differentially expressed proteins were associated with Ca 2+ homeostasis. Changes in ADP-ribosyl cyclase (ADPRC), lysophosphatidic acid receptor 3 (LPAR3), calreticulin, ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1), synaptosomal nerve-associated protein 25 (SNAP 25) and transgelin 3 protein

CD38 and MGluR1 as possible signaling molecules involved in epileptogenesis: a potential role for NAD+ homeostasis

Shima Khodaverdian, Elahe Dashtban-Moghadam, Bahareh Dabirmanesh, Javad Mirnajafi-Zadeh, Mohammad Taleb, Khosro Khajeh, Yaghoub Fathollahi
Journal PapersBrain Research , 2021 April 28, {Pages 147509 }

Abstract

In spite of long-term intensive scientific research efforts, there are still many issues concerning the mechanisms of epileptogenesis and epilepsy to be resolved. Temporal lobe, in particular hippocampus, is vulnerable to epileptogenic process. Herein, electrical kindling model of temporal lobe were analyzed using proteomic approach. A dramatic decrease in nicotinamide adenine dinucleotide (NAD+) level was exhibited during the kindling procedure in hippocampus. After stage 3, high CD38 expression was detected by qPCR, nano-liquid chromatography-tandem mass spectrometry (nano-LC-MS/MS) and western blot analysis. An increase in expression of CD38/NADase activity was observed during the kindling procedure in hippocampus that represent it as on

The application of the QDs/H2O2 chemiluminescence system in HRP assay and HRP-based immunoassay

F Ghavamipour, K Khajeh, RH Sajedi
Journal Papers , , {Pages }

Abstract

Extracting Potential New Targets for Treatment of Adenoid Cystic Carcinoma Using Bioinformatic Methods

TF Pordanjani, B Dabirmanesh, P Choopanian, M Mirzaie, S Mohebbi, ...
Journal Papers , , {Pages }

Abstract

In Vitro Cellular Uptake Studies of Self-Assembled Fluorinated Nanoparticles Labelled with Antibodies

M Atabakhshi-Kashi, M Carril, H Mahdavi, WJ Parak, C Carrillo-Carrion, ...
Journal Papers , , {Pages }

Abstract

Characterization of Interaction of the MBP-Tagged MuRif1-C-Terminal Domain with G-Quadruplex DNA by SPR

H Ghadiri, S Alavi, B Dabirmanesh, K Khajeh
Journal Papers , , {Pages }

Abstract

ИЗУЧЕНИЕ ВЗАИМОДЕЙСТВИЯ С-КОНЦЕВОГО ДОМЕНА БЕЛКА MuRif1 МЕЧЕННОГО MBP С G-КВАДРУПЛЕКСАМИ ДНК С ПОМОЩЬЮ …

H Ghadiri, S Alavi, B Dabirmanesh, K Khajeh
Journal Papers , , {Pages }

Abstract

Developing a Fluorescent Hybrid Nanobiosensor Based on Quantum Dots and Azoreductase Enzyme for Methyl Red Monitoring

F Hajipour, S Asad, MA Amoozegar, AA Javidparvar, J Tang, H Zhong, ...
Journal Papers , , {Pages }

Abstract

Interplay of isoform 1N4R tau protein and amyloid-β peptide fragment 25–35 in reducing and non-reducing conditions

F Mohammadi, Z Takalloo, H Rahmani, MA Nasiri Khalili, K Khajeh, ...
Journal Papers , , {Pages }

Abstract

Enhancing myelin repair in experimental model of multiple sclerosis using immobilized chondroitinase ABC I on porous silicon nanoparticles

Safoura Rezaei, Bahareh Dabirmanesh, Leila Zare, Abolfazl Golestani, Mohammad Javan, Khosro Khajeh
Journal PapersInternational Journal of Biological Macromolecules , Volume 146 , 2020 March 1, {Pages 162-170 }

Abstract

Removal of chondroitin sulfate proteoglycans (CSPGs) with chondroitinase ABC I (ChABC) facilitates axonal plasticity, axonal regeneration and remyelination, following injury to the central nervous system (CNS). However, the ChABC rapidly undergoes thermal inactivity and needs to be injected repeatedly. Here this limitation was overcame by immobilizing the ChABC on porous silicon (PSi) nanoparticles (ChABC@PSi). The efficacy of immobilized ChABC on CSPGs level and the demyelination insult was assessed in mice corpora callosa demyelinated by 6?weeks cuprizone (CPZ) feeding. ChABC@PSi was able to reduce the amount of CSPGs two weeks after animals treatment. CSPGs digestion by ChABC@PSi reduced the extent of demyelinated area as well as the ast

Examination of Chondroitinase ABC I Immobilization onto Dextran-coated Fe3O4 Nanoparticles and its in-vitro Release

Hossein Askaripour, Manouchehr Vossoughi, Khosro Khajeh, Iran Alemzadeh
Journal PapersJournal of Biotechnology , 2020 January 11, {Pages }

Abstract

Chondroitinase ABC I (cABC I) has received notable attention in treatment of spinal cord injuries and its application as therapeutics has been limited due to low thermal stability at physiological temperature. In this study, cABC I enzyme was immobilized on the dextran-coated Fe3O4 nanoparticles through physical adsorption to improve the thermal stability. The nanoparticles were characterized using XRD, SEM, VSM, and FTIR analyses. Response surface methodology and central composite design were employed to assess factors affecting the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 ?C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions. It was found

Molecular Dynamics Study of the Human Beta-defensins 2 and 3 Chimeric Peptides with the Cell Membrane Model of Pseudomonas aeruginosa

Mohammad Davoud Ghafari, Iraj Rasooli, Khosro Khajeh, Bahareh Dabirmanesh, Parviz Owlia
Journal PapersInternational Journal of Peptide Research and Therapeutics , 2020 January 1, {Pages 18-Jan }

Abstract

Evaluation of changes expression of D1, mGluR1 and CD38 genes after electrical kindling and treatment with low-frequency stimulation in rat

Khosro Khajeh, Javad Mirnajafi-Zadeh, Bahareh Dabirmanesh, Shima Khodaverdian
Journal PapersModares Journal of Biotechnology , Volume 11 , Issue 1, 2020 March 10, {Pages 0-0 }

Abstract

Evaluation of changes expression of D1 mGluR1 and CD38 genes after electrical kindling and treatment with low-frequency stimulation in rat-Tarbiat Modares University Journals System-Modares Journal of Biotechnology

Enhanced Sensitivity of VEGF Detection Using Catalase-Mediated Chemiluminescence Immunoassay Based on CdTe QD/H2O2 System

R Sajedi, F Ghavamipour, H Rahmani, M Shanehsaz, K Khajeh, M Mirshahi
Journal Papers , 2020 April 23, {Pages }

Abstract

Background Since vascular endothelial growth factor (VEGF) is a major regulator of cancer angiogenesis, it is essential to develop a technology for its sensitive detection. Herein, we sensitized a chemiluminescence (CL) immunoassay through the combination of H 2 O 2-sensitive TGA-CdTe quantum dot (QD) as signal transduction, dextran system as a cross-linker to prepare enzyme-labeled antigen and the ultrahigh bioactivity of catalase (CAT) as reporter enzyme. Results Under the optimized experimental conditions, the CL-ELISA method can detect VEGF in the excellent linear range of 2–35000 pg mL− 1, with a detection limit (S/N= 3) of 0.5 pg mL− 1 which was approximately 10 times lower than the commercial colorimetric immunoassay. This prop

Enhanced Sensitivity of VEGF Detection Using Catalase-Mediated Chemiluminescence Immunoassay Based on CdTe QD/H2O2 System

Fahimeh Ghavamipour, Hossein Rahmani, Maryam Shanehsaz, Khosro Khajeh, Manouchehr Mirshahi, Reza Sajedi
Journal Papers , 2020 April 23, {Pages }

Abstract

ResultsUnder the optimized experimental conditions, the CL-ELISA method can detect VEGF in the excellent linear range of 2–35000 pg mL− 1, with a detection limit (S/N= 3) of 0.5 pg mL− 1 which was approximately 10 times lower than the commercial colorimetric immunoassay. This proposed method has been successfully applied to the clinical determination of VEGF in the human serum samples and the results illustrated the excellent correlation with the conventional ELISA method (R2= 0.997). The suitable recovery rate of the method in the serum ranged from 97–107%, with a relative standard deviation of 1.2–13.4%.

دروس نیمسال جاری

  • دكتري
    ژنتيك مولكولي پيشرفته 2 ( واحد)
    دانشکده علوم زیستی، گروه ژنتيك
  • دكتري
    طراحي مهاركنندگان آنزيمي ( واحد)
  • دكتري
    خود بازيابي در سامانه هاي زيستي ( واحد)
  • كارشناسي ارشد
    زيست فناوري ( واحد)

دروس نیمسال قبل

  • كارشناسي ارشد
    آنزيم شناسي ( واحد)
    دانشکده علوم زیستی، گروه بيوشيمي
  • دكتر پریسا , نصراللهی
  • دكتر فهیمه , قوامی پور
  • 1397
    عزيزيان, فاطمه
    كلونينگ ، بيان ، تخليص و تعيين ويژگي آنزيم انتروكيناز در باكتري E.coli
  • 1397
    بحريني, فربد
  • 1398
    تاجيك باغخواص, فاطمه
  • 1398
    طبيب, سيدعليرضا
  • 1392
    شفيعي, سودابه
    ارزيابي تغييرات بياني پروتئوم اشك در بيماران مبتلا به قوز قرنيه به دنبال درمان با كراس لينك كلاژن
  • 1392
    غديري, حامد
  • 1393
    رنجبر روئين تن, سميرا
  • 1394
    خداورديان, شيما
  • 1394
    دشتبان مقدم, الهه سادات
  • 1394
    فروغي پردنجاني, طيبه
  • 1394
    علوي, ثنا
  • 1395
    گرانپايه واقعي, مرضيه
  • 1396
    صارمي, صابره
  • 1396
    ثابت, فرشته سادات
  • 1397
    محسني درگاه, مسعود
    داده ای یافت نشد
    داده ای یافت نشد

مهم

جدید

    اطلاعیه ای درج نشده است