En
  • دکتری (1386)

    بیولوژی ملکولی

    ژاپن، ژاپن

  • دکتری (1377)

    قارچ شناسی

    دانشگاه تربیت مدرس،

  • کارشناسی‌ارشد (1371)

    قارچ شناسی

    دانشگاه تربیت مدرس،

  • کارشناسی (1368)

    میکروبیولوژی

    دانشگاه اصفهان، ایران

  • بكارگيري فناوري نانو در تهيه تركيبات ضد قارچي از منابع طبيعي و دستيابي به فرمولاسيون درماني در درمان عفونتهاي قارچي در مدلهاي تجربي و سطح ملکولی
  • بررسي ملكولي پراكندگي عوامل قارچي بيماريزا و مولد سم جهت تعيين ژنوتايپينگ و مقاومت دارويي
  • بكارگيري نانوبيوسنسورها جهت رديابي و تشخيص عفونت هاي قارچي مهاجم
  • غربالگري، شناسايي و كلونينگ ژن هاي دخيل در توليد تركيبات ضد قارچي از منابع طبيعي
  • کشت قارچ

دکتر معصومه شمس قهفرخی در سال 1345 در تهران متولد شد. وی در سال 1368 موفق به اخذ مدرک لیسانس در رشته میکروبیولوژی از دانشگاه اصفهان گردید. در سال های 1371 و 1377 با اخذ مدرک کارشناسی ارشد و دکترای تخصصی (Ph.D.) از دانشکده علوم پزشکی دانشگاه تربیت مدرس فارغ التحصیل شد. پس از طی یک دوره 2 ساله فوق دکتری در زمینه قارچ شناسی ملکولی در کشور ژاپن در حال حاضر به عنوان عضو هیئت علمی تمام وقت دانشگاه تربیت مدرس مشغول به فعالیت می باشد و به انجام پروژه تحقیقاتی گروهی در خصوص طراحی روشهای تشخیص عوامل قارچی بیماریزا و مقاوم به دارو، بررسی ارتباطات ژنتیکی و فاکتورهای دخیل در بیماریزایی انها با استفاده از انواع تکنیک های ملکولی نظیر Sequencing، Real time-PCR، Multiplex-PCR، شناسایی و جداسازی ترکیبات ضد قارچی از منابع طبیعی (از جمله گیاهان و ارگانیسم ها) و بررسی آنها بر روی فعالیت های حیاتی قارچ های بیماری زا، ارزیابی اثرات ضدقارچی نانوذرات و مکانیسم تحویل دارو در سطح سلولی و مولکولی در سلول قارچ جهت یافتن ترکیبات ضد قارچی مناسب مشغول است. از ایشان علاوه بر چاپ کتب فارسی و انگلیسی در انتشارات معتبر بین المللی در زمینه قارچ شناسی پزشکی، مقالات متعددی در مجلات معتبر داخلی و خارجی به چاپ رسیده است.

ارتباط

رزومه

Antifungal activity and mechanism of action of dichloromethane extract fraction A from Streptomyces libani against Aspergillus fumigatus

Maryam Azish, Masoomeh Shams‐Ghahfarokhi, Mehdi Razzaghi‐Abyaneh
Journal PapersJournal of Applied Microbiology , 2021 February 15, {Pages }

Abstract

Aims This study aimed to investigate the mechanism of antifungal action of Streptomyces libani dichloromethane extract fraction A (DCEFA) against Aspergillus fumigatus and the host cytotoxicity. Methods and Results DCEFA was purified from S. libani by autobiography and showed strong antifungal activity against A. fumigatus. A combination of electron microscopy, cell permeability assays, total oxidant status (TOS) assay, cell cytotoxicity assay, and hemolysis activity were carried out to determine the target site of DCEFA. Exposure of A. fumigatus to DCEFA caused the damage to membranous cellular structures and increased release of cellular materials, potassium ions and TOS production. DCEFA was bound to ergosterol but did not affect fun

Inhibitory effects and mechanism of antifungal action of the natural cyclic depsipeptide, aureobasidin A against Cryptococcus neoformans

Mostafa Teymuri, Masoomeh Shams-Ghahfarokhi, Mehdi Razzaghi-Abyaneh
Journal PapersBioorganic & Medicinal Chemistry Letters , 2021 April 1, {Pages 128013 }

Abstract

Cryptococcosis is an opportunistic fungal infection caused mainly by Cryptococcus neoformans. The aim of the present study was to evaluate the inhibitory effect of aureobasidin A on C. neoformans with special focus on its mode of action. The effect of aureobasidin A on cell membrane ergosterol content, cell wall permeability, membrane pumps activities, the total oxidant status (TOS) and melanin production was evaluated. Cytotoxicity and cell hemolysis, and laccase (LacI) and β1,2-xylosyltransferase (Cxt1p) gene expression were also evaluated. Aureobasidin A reduced melanin production and increased extracellular potassium leakage at 0.5?MIC concentration. This peptide has no effect on fungal cell wall integrity. Cell membrane ergosterol con

Design, Recombinant Production and Biological Activity of a Plant-Derived Antifungal Peptide MCh-AMP1 in Escherichia coli

Sima Sadat Seyedjavadi, Soghra Khani, Raheleh Halabian, Mehdi Goudarzi, Hamideh Mahmoodzadeh Hosseini, Ali Eslamifar, Masoomeh Shams-Ghahfarokhi, Abbas Ali Imani Fooladi, Jafar Amani, Mehdi Razzaghi-Abyaneh
Journal PapersFrontiers in Fungal Biology , Volume 2 , 2021 January , {Pages 10 }

Abstract

Fungal species resistant to current antifungal agents are considered as a serious threat to human health; the dilemma that has dragged attentions toward other sources of antifungals such as antimicrobial peptides (AMPs). In order to improve biological activity of a recently described antifungal peptide MCh-AMP1 from Matricaria chamomilla flowers, MCh-AMP1dimer (DiMCh-AMP1), containing 61 amino acid residues connected by flexible linker (GPDGSGPDESGPDES) was designed, expressed in Escherichia coli and its structure was analyzed using bioinformatics tools. DiMCh-AMP1 synthetic gene was cloned into pET-28a expression vector, which was then used to transform E. coli BL21 (DE3) strain. His-tag purification was achieved using metal-chelate affini

Design, Dimerization, and Recombinant Production of MCh-AMP1–Derived Peptide in Escherichia coli and Evaluation of Its Antifungal Activity and Cytotoxicity

SS Seyedjavadi, S Khani, J Amani, R Halabian, M Goudarzi, HM Hosseini, A Eslamifar, M Shams-Ghahfarokhi, AA Imani Fooladi, M Razzaghi-Abyaneh
Journal PapersFront. Fungal Biol , Volume 2 , 2021 April 26, {Pages 638595 }

Abstract

Design, Dimerization, and Recombinant Production of MCh-AMP1–Derived Peptide in Escherichia coli and Evaluation of Its Antifun

Molecular Epidemiology, Genetic Diversity and Antifungal Susceptibility of Major Pathogenic Dermatophytes Isolated from Human Dermatophytosis

Zahra Salehi, Masoomeh Shams-Ghahfarokhi, Mehdi Razzaghi-Abyaneh
Journal PapersFrontiers in Microbiology , Volume 12 , 2021 January , {Pages 1145 }

Abstract

Background- Dermatophytes are a homogeneous group of species with low genetic diversity and there are still many uncertainties about the boundaries among species. Objectives- Aiming at clarifying the relationships among species in the genus and introducing suitable genes for multilocus sequence typing (MLST), a new MLST scheme approach was developed to characterize the major pathogenic dermatophytes. Methods- We performed maximum parsimony, MrBayes, RAxML, and eBURST analyses, based on the MLST scheme to scrutinize the evolution within 95 clinical isolates and 4 reference strains belonging to the four major dermatophytes species. Then, the discriminatory power, pairwise genetic distances, ratio dN/dS, and sequence types (STs) of these isola

Mycosynthesis and Physicochemical Characterization of Vanadium Oxide Nanoparticles Using the Cell-Free Filtrate of Fusarium oxysporum and Evaluation of Their Cytotoxic and …

M Gholami-Shabani, F Sotoodehnejadnematalahi, M Shams-Ghahfarokhi, ...
Journal Papers , , {Pages }

Abstract

The relationship between physical environment and work stress with job satisfaction

MS Ghahfarokhi, MR Ghasemi, FS Gahfarokhi
Journal Papers , , {Pages }

Abstract

Isolation, Characterization and Mode of Action of a Plant-Derived Antifungal Peptide, Cc-AFP1 Against Aspergillus Fumigatus

SS Seyedjavadi, S Khani, M Goudarzi, H Zare-Zardini, ...
Journal Papers , , {Pages }

Abstract

Molecular Epidemiology, Genetic Diversity, and Antifungal Susceptibility of Major Pathogenic Dermatophytes Isolated From Human Dermatophytosis

Z Salehi, M Shams-Ghahfarokhi, M Razzaghi-Abyaneh
Journal Papers , , {Pages }

Abstract

Effect of Allium cepa on LAC1 gene expression and physiological activities in Cryptococcus neoformans

SA Musavinasab-Mobarakeh, M Shams-Ghahfarokhi, ...
Journal Papers , , {Pages }

Abstract

Characterization, Biological Activity, and Mechanism of Action of a Plant-Based Novel Antifungal Peptide, Cc-AFP1, Isolated From Carum carvi

SS Seyedjavadi, S Khani, M Goudarzi, H Zare-Zardini, ...
Journal Papers , , {Pages }

Abstract

Antifungal Activity, Cytotoxicity and Mechanism of Action of Nitroheteroaryl-1, 3, 4-thiadiazole Containing N-benzyl and N-methoxyethyl Substitution Against Aspergillus fumigatus

P MORADABADI, A TAHGHIGHI, L JALILI-BALEH, A FOROUMADI, ...
Journal Papers , , {Pages }

Abstract

Internal Transcribed Spacer rDNA and TEF-1α Gene Sequencing of Pathogenic Dermatophyte Species and Differentiation of Closely Related Species Using PCR-RFLP of The?…

Zahra Salehi, Masoomeh Shams-Ghahfarokhi, Mehdi Razzaghi-Abyaneh
Journal PapersCell Journal (Yakhteh) , Volume 22 , Issue 1, 2020 April 1, {Pages }

Abstract

Objective: Precise identification of dermatophyte species significantly improves treatment and controls measures of dermatophytosis in human and animals. This study was designed to evaluate molecular tools effectiveness of the gene sequencing and DNA-based fragment polymorphism analysis for accurate identification and differentiation of closelyrelated dermatophyte species isolated from clinical cases of dermatophytosis and their antifungal susceptibility to the current antifungal agents. Materials and Methods: In this experimental study, a total of 95 skin samples were inoculated into mycobiotic agar for two weeks at 28 C. Morphological characteristics of the isolated dermatophytes were evaluated. DNA was extracted from the fungal culture f

Antifungal Nanotherapy: A Novel Approach to Combat Superficial Fungal Infections

Farnoush Asghari-Paskiabi, Zahra Jahanshiri, Masoomeh Shams-Ghahfarokhi, Mehdi Razzaghi-Abyaneh
Journal Papers , 2020 January , {Pages 93-107 }

Abstract

Superficial fungal infections (SFIs) affect up to 25% of population all over the world. Although dermatophytosis is the main SFIs with worldwide distribution, tinea versicolor caused by Malassezia species and Candida -related infections are also common. SFIs have diverse etiologic agents, which differ in pathogenesis and geographic distribution with increasing rate of resistant species to current antifungal therapy. Nowadays, the conventional antifungal therapy of SFIs using current antifungals of azoles, allylamines, and griseofulvin have some drawbacks like liver toxicity, skin problem, severe headaches and sometimes recurrences and drug–drug interactions especially in patients who are under drug treatment for other dise

Antifungal activity of eugenol on Cryptococcus neoformans biological activity and Cxt1p gene expression

Parviz Hassanpour, Masoomeh Shams-Ghahfarokhi, Mehdi Razzaghi-Abyaneh
Journal PapersCurrent Medical Mycology , 2020 February 16, {Pages }

Abstract

Materials and Methods: For the purpose of the study, the growth, urease, synergism activity, and disk diffusion of C. neoformans were assessed in eugenol-treated culture. The minimum inhibitory concentration (MIC) was determined by the Clinical and Laboratory Standards Institute M27-A3 method at a concentration range of 0.062-2 mg/mL. Subsequently, the expression of Cxt1p genes was studied at the MIC50 concentration of eugenol using real-time polymerase chain reaction. Results: The obtained results showed that eugenol at the concentrations of 125 and 500 ?g/mL resulted in 50% and 100% growth inhibition in C. neoformans, respectively. In terms of urease activity, the results showed that the addition of MIC50 of eugenol and fluconazole to ure

In vivo and in vitro Pathogenesis and Virulence Factors of Candida albicans Strains Isolated from Cutaneous Candidiasis

Golnar Sadeghi, Seyed Fazllolah Mousavi, Mina Ebrahimi-Rad, Esmat Mirabzadeh-Ardekani, Ali Eslamifar, Masoomeh Shams-Ghahfarokhi, Zahra Jahanshiri, Mehdi Razzaghi-Abyaneh
Journal PapersIranian Biomedical Journal , 2020 January , {Pages 0-0 }

Abstract

Background: The Candida albicans is one of the most important global opportunistic pathogens, and the incidence of candidiasis has increased over the past few decades. Despite the established role of skin in defense against fungal invasion, little has been documented about the pathogenesis of Candida species when changing from normal flora to pathogens of vaginal and gastrointestinal epithelia. This study was carried out to determine in vivo and in vitro pathogenesis of clinical C. albicans strains isolated from skin lesions Methods: In this study, association of in vivo and in vitro pathogenesis of C. albicans isolates with different evolutionary origins was investigated. Oral and systemic experimental candidiasis was established in BALB/C

Effect of Carum carvi essential oil on ERG6 gene expression and virulence factors in Candida albicans

Samira Nasiri, Masoomeh Shams-Ghahfarokhi, Mehdi Razzaghi-Abyaneh
Journal PapersCurrent Medical Mycology , 2020 July 7, {Pages }

Abstract

Background and Purpose: The present study was conducted to investigate the inhibitory effects of Carum carvi essential oil (EO) against ERG6 gene expression in relation to fungal growth and some important virulence factors in Candida albicans.Materials and Methods: The minimum inhibitory concentration (MIC) of C. carvi EO against C. albicans was determined by the Clinical and Laboratory Standards Institute M27-A4 method at a concentration range of 20-1280 μg/ml. Furthermore, the expression of ERG6 gene was studied at the 0.5? MIC concentration of C. carvi EO using real-time polymerase chain reaction. The proteinase and phospholipase activities, cell surface hydrophobicity (CSH), and cell membrane ergosterol (CME) content of C. albicans wer

Carum carvi essential oil suppresses ERG6 gene expression and affects virulence factors in Candida albicans

Samira Nasiri, Masoomeh Shams-Ghahfarokhi, Mehdi Razzaghi-Abyaneh
Journal PapersCurrent Medical Mycology , Issue Articles in Press, 2020 July 7, {Pages }

Abstract

Not ready for print

Antibiofilm Activity of Cellobiose Dehydrogenase Enzyme (CDH) Isolated from Aspergillus niger on Biofilm of Clinical Staphylococcus epidermidis and Pseudomonas aeruginosa Isolates

Ramin Rasouli, Masoumeh Navidinia, Masoomeh Shams Ghahfarokhi, Rouhollah Vahabpour Roudsari, Saadat Adabian, Ahmad Reza Baghestani
Journal PapersArchives of Clinical Infectious Diseases , Volume 15 , Issue 1, 2020 February 29, {Pages }

Abstract

Background: It is estimated that more than 80% of cases of human infections are related to biofilm formation by invasive bacteria. So, in this study, we considered the activity of cellobiose dehydrogenase enzyme (CDH) isolated from Aspergillus niger, as an antibiofilm agent, on biofilm of clinical Staphylococcus epidermidis and Pseudomonas aeruginosa isolates. Methods: In this study, five standard strains of Aspergillus niger were purchased for CDH production. Of the 42 isolated bacterial strains, 24 strains were Staphylococcus epidermidis and 18 strains were Pseudomonas aeruginosa. Zymogram method was used for screening of CDH. The CDH activity was measured by monitoring the decrease in absorbance of 2, 6-dichlorophenolindophenol (DCPIP) s

Optimization of the antifungal metabolite production in Streptomyces libani isolated from northern forests soil in Iran

Maryam Azish, Masoomeh Shams-Ghahfarokhi, Mehdi Razzaghi-Abyaneh
Journal PapersCurrent Medical Mycology , 2020 November 22, {Pages }

Abstract

Abstract Background and Objectives: Soil bacteria have extreme population diversity among natural sources and are able to produce a wide array of antifungal metabolites. This study aimed toisolate and identifythebioactive metabolite-producing bacteria from forest soils and evaluate their antimicrobial potent against some pathogenic organisms. Materials and Methods:In this study, soil samples werescreened?for antifungal activity against?Aspergillus fumigatuson glucose-yeast extract (GY) agar using a visual agar plate assay method. All growing bacteria were examined for antifungal activity, and antagonistic bacteria were identified based on 16S ribosomal RNA sequence analysis. For optimization ofthe production of antifungal bioactive metaboli

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دروس نیمسال جاری

  • دكتري
    داروهاي ضد قارچي و مكانيسم اثر آنها ( واحد)
    دانشکده علوم پزشکی، گروه قارچ شناسي
  • دكتري
    داروهاي ضد قارچي و مكانيسم اثر آنها ( واحد)
  • كارشناسي ارشد
    زيست شناسي سلولي و مولكولي در قارچ شناسي پزشكي ( واحد)
  • كارشناسي ارشد
    زيست شناسي سلولي و مولكولي در قارچ شناسي پزشكي ( واحد)
  • كارشناسي ارشد
    بيماري هاي قارچي زيرجلدي ( واحد)
  • كارشناسي ارشد
    بيماري هاي قارچي زيرجلدي ( واحد)

دروس نیمسال قبل

  • دكتري
    مايكوتوكسين ها و مايكوتوكسيكوزيس ( واحد)
    دانشکده علوم پزشکی، گروه قارچ شناسي
  • دكتري
    بيماريهاي پوست ( واحد)
  • دكتري
    بيماريهاي پوست ( واحد)
  • كارشناسي ارشد
    مقدمات قارچ شناسي پزشكي ( واحد)
  • كارشناسي ارشد
    مقدمات قارچ شناسي پزشكي ( واحد)
  • دكتري
    بيوانفورماتيك ( واحد)
  • دكتري
    بيوانفورماتيك ( واحد)
  • 1400
    رهبري, فاطمه
    بررسي تاثير نانوامولسيون كوئرستين انكپسوله شده با بتا سيكلودكسترين/سوكسينات در تشكيل بيوفيلم و بيان ژن ALS3 دخيل در تشكيل ان در سويه هاي كانديدا آلبيكنس جدا شده از كانديدمي
  • 1397
    اميري زاد, كاظم
    جداسازي و شناسايي عوامل كانديدايي دركودكان بستري در بيمارستان مركز طبي كودكان و ارزيابي بيان ژنهاي درگير در مقاومت هاي دارويي و بروز موتاسيون در آنها
  • 1400
    موقر, راضيه
  • 1400
    محمود, حسين رياض
  • عضو بین المللی هیئت تحریریه IRJPS
  • مدیر گروه قارچ شناسی
  • عضو هیئت تحریریه مجله قارچ شناسی پزشکی پیشرفته
  • عضو بین المللی هیئت تحریریه IRJPS
  • عضویت در هیات تحریریه مجله سر حدها در میکروبیولوژی
  • عضویت در هیات تحریریه مجله journal of frontiersin microbiology سر حدها در میکروبیولوژی
  • عضویت در هیات تحریریه نشریه Current Medical Microbiology قارچ شناسی پزشکی
  • عضویت در هیئت تحریریه مجله Micro & Nano Biomedicine
  • عضو هیئت تحریریه مجله ARC Journal of Pharmaceutical Sciences (ajps)
  • عضویت در هیات تحریریه Journal of Biological Sciences
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