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  • Metabolic Engineering

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Evaluation of Response of Common Bean Cultivars with Two Different Growth Types under Weed Competition

Seyede Zahra Tabatabaiepour, Zahra Tahmasebi, Alireza Taab, Sajad Rashidi Monfared
Journal PaperIranian Journal Pulses Research , 2021 May 22, {Pages }

Abstract

Introduction: Common bean (Phaseolus vulgaris L.) is an important source of protein and calorie. This plant plays an important role in supplying the human's protein. Common bean is highly susceptible to weeds due to low growth rate early in the growing season. Therefore, competition with weeds causes a significant reduction in grain yield at the end of the growing season. Weed competition is a stress on plants. Weeds and crops compete for nutrients, water, light and space (CO2). By examining the effect of time of weed interference during the critical period, valuable information can be obtained from the physiological base of competition between weeds and crops. Common bean varieties, with different morphological and genetic characteristics,

Field-grown donor plants and arabinogalactan proteins improve microspore embryogenesis in sweet pepper (Capsicum annuum L.)

Azam Pourmohammad, Ahmad Moieni, Hamid Dehghani, Sajad Rashidi Monfared
Journal PaperIn Vitro Cellular & Developmental Biology-Plant , 2021 March 8, {Pages 09-Jan }

Abstract

An effective culture system for producing embryos was developed from isolated microspores of sweet pepper (Capsicum annuum L.). The present work aimed to define exogenous arabinogalactan proteins (AGPs) on the embryogenesis of sweet pepper under the growth environments of donor plants. The experiments were performed with the cultivars Arancia, California wonder, Californium, Camelot, Inspiration, Plato, and Taranto under three different growth environments of the donor plants. The impacts of the different numbers of sweet pepper ovaries (0, 5, 10, and 15) were evaluated on microspore embryogenesis of cultivars in a factorial design with five replications. The simultaneous application of 10 ovaries in stress (sucrose starvation; temperature)

Improving water deficit tolerance of Salvia officinalis L. using putrescine

Maryam Mohammadi-Cheraghabadi, Seyed Ali Mohammad Modarres-Sanavy, Fatemeh Sefidkon, Sajad Rashidi-Monfared, Ali Mokhtassi-Bidgoli
Journal Paper , 2021 April 21, {Pages }

Abstract

To study the effects of foliar application of putrescine (distilled water (0), 0.75, 1.5, and 2.25 mM) and water de cit stress (20%, 40%, 60%, and 80% available soil water depletion (ASWD)) on the physiological, biochemical, and molecular attributes of sage, a factorial experiment was performed in a completely randomized design with three replications in the growth chamber. The results of qRT-PCR analysis showed that putrescine concentration, irrigation regime, and the two-way interaction between irrigation regime and putrescine concentration signi cantly in uenced cineole synthase, sabinene synthase, and bornyl diphosphate synthase relative expression. The highest concentration of 1, 8-cineole, camphor, αthujone, β-thujone, cineole synth

Toward understanding of the methoxylated flavonoid biosynthesis pathway in Dracocephalum kotschyi Boiss

A Poursalavati, S Rashidi-Monfared, A Ebrahimi
Journal Paper , , {Pages }

Abstract

Identification and in-silico characterization of taxadien-5α-ol-O-acetyltransferase (TDAT) gene in Corylus avellana L.

M Raeispour Shirazi, SA Rahpeyma, S Rashidi Monfared, J Zolala, ...
Journal Paper , , {Pages }

Abstract

Population and individual multivariate analysis of white (Morus alba), red (Morus rubra) and black (Morus nigra) mulberry genotypes: applications for breeding, conservation and …

A Ebrahimi, A Poursalavati, M Mohamadi Esboei, SR Monfared, M Sahebi, ...
Journal Paper , , {Pages }

Abstract

The effect of 24-epi-brassinosteroid on the expression of some genes involved in the diosgenin biosynthetic pathway of fenugreek (Trigonella foenum-graecum L.) under high …

S Sheikhi, A Ebrahimi, P Heidari, MR Amerian, S Rashidi Monfared
Journal Paper , , {Pages }

Abstract

Evaluation of response of Red bean cultivars with two different growth habits on weed competition

SZ Tabatabaiepour, Z Tahmasebi, A Taab, S Rashidi Monfared
Journal Paper , , {Pages }

Abstract

Evaluation of response of Red bean cultivars with two different growth habits on weed competition

SZ Tabatabaiepour, Z Tahmasebi, A Taab, S Rashidi Monfared
Journal Paper , , {Pages }

Abstract

Comprehensive functional analysis and mapping of SSR markers in the chickpea genome (Cicer arietinum L.)

AliAkbar Asadi, Amin Ebrahimi, Sajad Rashidi-Monfared, Mohammad Basiri, Javad Akbari-Afjani
Journal PaperComputational Biology and Chemistry , Volume 84 , 2020 February 1, {Pages 107169 }

Abstract

Plant molecular breeding largely depends on the relationship between molecular markers and major traits. Herein, a total of 32,962 genomic simple sequence repeats (SSRs) were detected in the whole genome of chickpea with an average density of 94.93 SSRs/Mb. Chickpea chromosomes uniformity test indicated that the genomic SSRs (gSSRs) were steadily distributed across the genome. Moreover, 48,667 transcriptome sequences were analyzed and 1949 SSR-containing transcript assembly contigs (TACs) were identified. The analysis showed that di- and trinucleotide SSRs were the most frequent SSR motifs within the transcriptome sequences. Among them, AT and TTA and AG and TTC motifs within the transcriptome showed the highest frequencies among di- and tr

Improving diosgenin production and its biosynthesis in Trigonella foenum-graecum L. hairy root cultures

Farnaz Zolfaghari, Sajad Rashidi-Monfared, Ahmad Moieni, Davar Abedini, Amin Ebrahimi
Journal PaperIndustrial Crops and Products , Volume 145 , 2020 March 1, {Pages 112075 }

Abstract

Induction of hairy roots stemmed from Agrobacterium rhizogenes transformation, is a key step to reach mass production of secondary metabolites in medicinal plants. Accordingly, this study is aimed to investigate potential impact of different A. rhizogenes strains, including ATCC15834, R1000, A4 and C58, on diosgenin biosynthesis in high- “Boshruyeh” (23.8 mg/gDW) and low “Hamadan” (6.4 mg/gDW) diosgenin-producing genotypes of fenugreek. Interestingly, comparing the metabolite extracted from both leaves and hairy roots of these genotypes suggested that hairy roots are promising platform to produce scalable amount of diosgenin, producing 143.96 mg/gDW in high diosgenin producing hairy roots (A4-mediated hairy roots induction in

Evaluation of phenotypic diversity of the endangered orchid (Orchis mascula): Emphasizing on breeding, conservation and development

Amin Ebrahimi, AliAkbar Asadi, Sajad Rashidi Monfared, Mohammad Sahebi, Sadra Rezaee, Yasin Khaledian
Journal PaperSouth African Journal of Botany , Volume 132 , 2020 August 1, {Pages 304-315 }

Abstract

The phenotypic diversity of the Iranian orchids has sharply decreased during the last 15 years and is currently threatened with local extinction and global climatic changes. Harvest of tuberous orchids has recently been raised in Iran owing to the high prices of orchid's tubers and international demand from neighboring countries. This has caused competition among orchid harvesters in the country so that the orchids are not even allowed to produce seeds. The present study was done aimed at protecting and identifying the phenotypic diversity within Orchis mascula genotypes in Abr forest, which is a natural habitat of Orchis mascula in Shahrood area, Northeast Iran. The morphological variability of 101 collected samples of early purple orchids

Identification and expression analysis of S-alk(en)yl-L-cysteine sulfoxide lyase isoform genes and determination of allicin contents in Allium?species

Vahid Sayadi, Ghasem Karimzadeh, Sajad Rashidi Monfared, Mohammad Reza Naghavi
Journal PaperPlos one , Volume 15 , Issue 2, 2020 February 24, {Pages e0228747 }

Abstract

Alliinase is the key enzyme in allicin biosynthesis pathway. In the current study, the identification and sequencing of alliinase genes along with determination of allicin contents were reported for Allium species with a novel report for Iranian endemic species. The presence of different isoforms in the Allium being discovered for the first time. In bulbs tissue, the highest allicin concentration was in Allium sativum, A. umbilicatum, and A. fistolosum (1.185%, 0.367%, and 0.34%, respectively), followed by A. spititatum (0.072%), A. lenkoranicum (0.055%), A. atroviolaseum (0.36%), A. rubellum (0.041%), and A. stamineum (0.007%). The highest allicin content in the leaves and roots were in A. sativum (0.13%), and A. stamineum (0.195%), respec

Molecular characterization and expression pattern of RhPAR, RhMYB1 and RhANS genes involving in scent and color production in Rosa damascena

Omid Rasouli, Nima Ahmadi, Sajad Rashidi Monfared
Journal PaperScientia Horticulturae , Volume 272 , 2020 October 15, {Pages 109399 }

Abstract

The aim of this study was to evaluate molecular and phytochemical changes in four landraces of Rosa damascena with different in color and floral developmental stages. Data showed the highest amount of anthocyanin content in stage B, while it was reduced at stage C during flower development in all landraces. Interestingly, increasing in cellular pH was occurred gradually in all landraces during flower development. Based on essential oil analysis, 2-Phenylethanol was detected in all landraces, except for Lavasanat. Molecular expression pattern of RhPAR gene showed high expression rate in all landraces at stage B with 6 time more than its control, except for Lavasanat. Similarly, the change in the expression rate was occurred in RhMYB1 and the

The Comparison of Assembly Softwares and Gene Ontology Analysis using transcriptome sequencing data from Dracocephalum kotschyi Boiss.

Abolnaser Poursalavati, Amin Ebrahimi, Sajad Rashidi Monfared
Journal PaperJournal of Cellular and Molecular Research (Iranian Journal of Biology) , Volume 33 , Issue 2, 2020 June 21, {Pages 207-217 }

Abstract

With fast advances in next generation sequencing technologies, they has become powerful and low-cost tools for transcriptome studies, Nowadays; de novo assembly of transcriptome data, has caused the formation of the new pathway in the study of non-model genome species. With the expansion of this technology and increasing the number of assembly softwares, choosing the best software for assembling transcriptome sequencing data has become a challenge for biologists. For the first time in this study, we used transcriptome sequencing data of Dracocephalum kotschyi in order to reach the appropriate parameters and superior software; so here we used Oases-velvet, SOAPdenovo-Trans, Trans-ABySS and Trinity softwares with two different values of K par

Identification and expression analysis of S-alk(en)yl-L-cysteine sulfoxide lyase isoform genes and determination of allicin contents in Allium species

V Sayadi, G Karimzadeh, S Rashidi Monfared, MR Naghavi
Journal Paper , , {Pages }

Abstract

Improving biomass and chicoric acid content in hairy roots of Echinacea purpurea L.

Reza Sajadi Tabar, Ahmad Moieni, Sajad Rashidi Monfared
Journal PaperBiologia , 2019 January , {Pages 11-Jan }

Abstract

Echinacea purpurea L. (2n = 2x = 22), is one of the most popular medicinal plants in developed countries containing important secondary metabolites including caffeic acid derivatives, amongst which chicoric acid is the most significant. Chicoric acid has positive effects on human immune system. Hairy roots culture of E. purpurea is an efficient method for producing chicoric acid, because of their rapid growth and higher chicoric acid content compared to an intact plant. In this research, the effects of simultaneous changes in [sucrose and NH4NO3] and [sucrose and Ca(NO3)2] concentrations, as well as the effect of change in KH2PO4 concentration, were studied on the dry weight (DW) of diploid hairy roots of Echinacea purp

Regulation of dedifferentiation and differentiation in different explants of Papaver rhoeas L. by one-step culture

Zahra Aghaali, Yoichiro Hoshino, Sajad Rashidi Monfared, Ahmad Moeini
Journal PaperScientia Horticulturae , Volume 246 , 2019 February 27, {Pages 366-370 }

Abstract

The corn poppy (Papaver rhoeas L.) is an important horticultural plant and one of the most well-known members of the Papaveraceae. The species is also used for the treatment of various diseases in folk medicine. Using a biotechnological approach, this species can be further improved. However, the corn poppy is a recalcitrant plant species for tissue culture. Here, we investigated what effects explant type and different auxins and cytokinins had on callus induction and shoot formation. Root, hypocotyl, cotyledon, petiole, and leaf explants were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of the auxins picloram, naphthaleneacetic acid, and 2,4-dichlorophenoxyacetic acid (0.5 and 1.0?

New insights into diosgenin biosynthesis pathway and its regulation in Trigonella foenum‐graecum L.

Mohammad Mohammadi, Tooba Mashayekh, Sajad Rashidi‐Monfared, Amin Ebrahimi, Davar Abedini
Journal PaperPhytochemical Analysis , 2019 August 30, {Pages }

Abstract

Introduction Throughout history, thousands of medicinal and aromatic plants have been widely utilised by people worldwide. Owing to them possessing of valuable compounds with little side effects in comparison with chemical drugs, herbs have been of interest to humans for a number of purposes. Diosgenin, driven from fenugreek, Trigonella foenum‐graecum L., has extensively drawn scientist's attention owing to having curable properties and being a precursor of steroid hormones synthesis. Nonetheless, complete knowledge about the biosynthesis pathway of this metabolite is still elusive. Objective In the present research, we isolated the full‐length CDS of 14 genes involving in diosgenin formation and measured their expression rate in va

The effects of promoter variations of the N-Methylcanadine 1-Hydroxylase (CYP82Y1) gene on the noscapine production in opium poppy

Davar Abedini, Sajad Rashidi Monfared, Alireza Abbasi
Journal PaperScientific reports , Volume 8 , Issue 1, 2018 March 21, {Pages 4973 }

Abstract

Noscapine is an antitumor alkaloid produced in opium poppy (Papaver somniferum) and some members of the Papaveraceae family. It has been primarily used for its antitussive effects; more recently, its anticancer properties were shown. Herein, we detected an SSR embedded in the promoter region of the CYP82Y1 gene, which was found to be the first committed-step enzyme in the noscapine biosynthesis pathway, using the MISA program. Some collected ecotypes of P. somniferum were investigated for understanding of SSRs role in the regulation of gene expression and metabolite content. Quantitative PCR showed that a variation in the motif repeat number (either a decrease or increase) down-regulated the expression of the CYP82Y1 gene. Furthermore, the

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