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Research field: Growth Factors, Bioactive Scaffolds and Nanobodies
Expert: Ms. Hafezeh Alizadeh
Phone: 00982182884484
Address: -
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In this study, poly (ε‐caprolactone) (PCL) scaffolds were printed and reinforced, simultaneously, with biodegradable poly glycolic acid (PGA) suture yarn, as a continuous reinforcing fiber, in the Fused Deposition Modeling (FDM) 3D printing process. Albeit PCL is a suitable material for biomedical applications, its low mechanical properties, and low degradation rate have limited its usage. A biocompatible suture yarn was used as the reinforcing material to enhance the mechanical properties and biodegradation characteristics, via an innovative method of continuous fiber embedding in the FDM process. The reinforced PCL samples were 3D printed with the setting porosity value of 60% and 0?/60?/120? lay-down pattern. The mechanical and biolog
Purpose The present study sought to design a multi‐functional fusion peptide with hydroxyapatite (HA) binding domain (HABD) and heparin binding domain (HBD). Methods The 74 amino acid fusion peptide contained N‐terminus of the fibrinogen β chain (β 15‐66), double G4S‐linker and 12 residues with HA affinity. This construct was designed, synthesized and cloned into pET21a(+) vector and expressed in E.coli. Results HABD facilitated purification of the fusion peptide by HA affinity chromatography. Kinetic peptide binding and release on HA scaffold showed sustained release of peptide for up to 16 days. Competitive ELISA and intrinsic fluorescence assays were applied to determine HBD affinity to bone morphogenetic protein (BMP)?
In the present study, Poly l-lactic acid (PLLA) resin compatible with digital light processing (DLP) 3D printing method was synthesized to produce hard tissue scaffolds. PLLA has been chosen as a decent material to mimic biological structures due to its relatively high strength as well as proper biocompatibility and biodegradation rate. After synthesis and functionalization of PLLA, using a facile method, porous models with 600-micron pore size and 70 % nominal porosity were designed and fabricated via DLP technique in order to investigate the effects of the two process parameters, light exposure time and dye concentration, on compressive strength and morphological features of the printed samples. The experimental results were then reconcil
Purpose The present study sought to design a multi‐functional fusion peptide with hydroxyapatite (HA) binding domain (HABD) and heparin binding domain (HBD). Methods The 74 amino acid fusion peptide contained N‐terminus of the fibrinogen β chain (β 15–66), double G4S‐linker and 12 residues with HA affinity. This construct was designed, synthesized and cloned into pET21a(+) vector and expressed in E. coli. Results HABD facilitated purification of the fusion peptide by HA affinity chromatography. Kinetic peptide binding and release on HA scaffold showed sustained release of peptide for up to 16 days. Competitive ELISA and intrinsic fluorescence assays were applied to determine HBD affinity to bone morphogenetic protein‐2 (
The original version of this article unfortunately contained an error in author group. The given name and family name was swapped erroneously for co-author. The correct name is Alireza Moshaverinia instead it was published incorrectly as Moshaverinia Alireza. The original article has been corrected.
IntroductionThis study aims to develop and characterize the regenerative potential of an atelopeptidized treated dentin matrix xenograft using in?vitro and in?vivo models.MethodsFreshly extracted bovine dentin was pulverized into 250- to 500-μm particles and demineralized with 17% EDTA for 1, 7, and 13 days. The samples were atelopeptidized with pepsin. The degree of demineralization and the effect of atelopeptidization were assessed using field emission scanning electron microscopy combined with energy-dispersive X-ray spectroscopy and Fourier transform infrared spectroscopy, respectively. The expression of dentin matrix acidic phosphoprotein 1, dentin sialophosphoprotein, and osteopontin was evaluated in dental pulp stem cells using quan
Because of the critical role of vascular endothelial growth factor (VEGF) in angiogenesis and its significantly increased serum levels in early stages of cancer, VEGF is considered an important prognostic biomarker in different cancers. Herein, the amplification power of PCR combined with phage displaying anti-VEGF VHH, a sensitive real-time immunoassay, was precisely designed based on phage display-mediated immuno-PCR (PD-IPCR) for the detection of VEGF. This system benefits from strong and specific binding of antigen and antibody in a sandwich immunosorbent assay platform using avastin (anti-VEGF monoclonal antibody) as the capture antibody. The anti-VEGF phage particles were used as both anti-VEGF agent and DNA template in the PD-IPCR. A
Background Due to the unique features of xenografts including large supply from donors, minimal risk of human disease transmission, and the lower cost of preparation and production compared to autografts and allografts, they are considered as attractive alternatives to traditional bone grafts. The animal source accessibility and production process have a direct correlation with the cost and quality of the final product. To evaluate whether the animal source of the bone has any effect on the physicochemical and histological properties of the final xenograft, three deproteinized bone grafts were prepared from sources that are easily available in Iran, including the bovine (DBB), camel (DCB), and ostrich (DOB). Methods In the current study
Human alpha1-antitrypsin is a glycoprotein comprised of 394 amino acids and 52 kDa molecular weight, which is mostly synthesized and secreted by hepatocytes, diffuses to interstitial lung tissues, and has an essential function to protected tissues against neutrophil elastase. One of the significant challenges in dealing with alpha-1-antitrypsin is the structural instability of the folded form of protein and, consequently, the accumulation of polymers in lung tissue. This makes patients vulnerable to chronic obstructive pulmonary disease, severe asthma, and emphysema. Intravenous augmentation therapy of alpha 1-antitrypsin is one of the most prevalent therapies. Moreover, patients who are candidates for that have respiratory symptoms, and th
The main aim of this study was to assess the influence of adding BCP particles on enhancing essential properties of PLLA matrix scaffolds for bone grafting purpose. Poly L-lactic acid (PLLA)/biphasic calcium phosphate (BCP) scaffolds were fabricated via a digital light processing (DLP) 3D printer, with 70% porosity and 600 ?m pore size. DLP method was utilized to generate scaffolds with sophisticated geometry, and the bio-composite material was introduced to benefit from positive aspects of polymeric/inorganic substances for bone regeneration. The selected BCP contents were 22.5, and 45?wt% compared with the control specimens with no content. Morphology, exact amount of BCP concentration as well as their distribution were assessed using com
Background: Anthrax is a zoonotic disease caused by Bacillus anthracis and it can be deadly in 6?days. Considerable efforts have been conducted toward developing more effective veterinary and human anthrax vaccines because these common vaccines have several limitations. B. anthracis secretes a tripartite toxin, comprising protective antigen (PA), edema factor (EF), and lethal factor (LF). Several studies have shown important role of PA in protection of anthrax. LF and EF induce production of toxin neutralizing antibodies too. PA in fusion form with LF/EF has synergistic effects as a potential subunit vaccine.Methods: In this study, for the first time, a triple chimeric protein called ELP was modeled by fusing three different domains of anth
Askari S. 1 MSc, Hasannia S.* 1 PhD, Hassan Sajedi R. 1 PhD, Yassaee VR 2 PhD
Background: Platelet‐rich fibrin (PRF) membranes can preserve alveolar ridge dimension after tooth extraction. Thus, it can be presumed that PRF suppresses the catabolic events that are caused by osteoclastic bone resorption.Methods: To address this possibility, we investigated the impact of soluble extracts of PRF membranes on in vitro osteoclastogenesis in murine bone marrow cultures. Osteoclastogenesis was induced by exposing murine bone marrow cultures to receptor activator of nuclear factor kappa B ligand (RANKL), macrophage colony‐stimulating factor (M‐CSF) and transforming growth factor‐beta 1 (TGF‐β1) in the presence or absence of PRF. Osteoclastogenesis was evaluated based on histochemical, gene expression and resorption
Objectives: Platelet‐rich fibrin (PRF) serves as a reservoir of bioactive molecules to support wound healing and bone regeneration. The beneficial action of PRF might involve macrophage polarization from pro‐inflammatory M1 towards pro‐resolving M2 phenotypes. This study aims to evaluate the effect of PRF on macrophage polarization.MethodsMurine primary macrophages and RAW264. 7 cells were exposed to saliva and lipopolysaccharides (LPS) with and without PRF lysates obtained by repeated freeze‐thawing or the secretome of PRF membranes, termed PRF conditioned medium. The expression of the M1 marker genes interleukin 1β (IL1β) and interleukin 6 (IL6) along with the M2 markers arginase‐1 and chitinase‐like 3 (Chil3 or YM1) were ev
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