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Background: The aim of the present study was to investigate the effect of Sodium Selenite (SS) supplemented media on oocyte maturation, expression of mitochondrial transcription factor A (TFAM) and embryo quality.Methods: Mouse Germinal Vesicle (GV) oocytes were collected after administration of Pregnant Mare Serum Gonadotropin (PMSG); in experimental group 1, oocytes were cultured and then subjected for in vitro maturation in the absence of SS, and in experimental group 2, they were matured in vitro in the presence of 10 ng/ml of SS up to 16 hr. The control group included MII oocytes obtained from the fallopian tubes after ovarian stimulation with PMSG, followed by human chorionic gonadotropin. Then, the expression of TFAM in MII oocytes i
Currently, one of the new therapeutic strategies is injection of hydrogel and cells to myocardial infarction (MI) patients, which has some limitations such as lack of electromechanical properties and neovascularization. In this study, we investigated the therapeutic potential of new electroactive hydrogel [Reduced graphene oxide (rGO)/Alginate (ALG)] encapsulated human bone marrow mesenchymal stem cell (BMSC) in different experimental groups. The study was done in rat model of chronic ischemic cardiomyopathy by ligating the left anterior descending coronary artery (LAD). Echocardiograms were analyzed at 4 and 8 weeks after MI induction.Experimental groups particularly (BMSC) encapsulated in rGO-ALG increased signi cantly improvement of frac
Background: The purpose of this study was to determine the effects of alginate hydrogel as a capsule to protect the ovary against possible detrimental effects of vitrification and warming on morphology and expression of apoptosis-related genes in the mouse ovary.Methods: In this experimental study, the ovaries from twenty-five female 8-week-old mice were divided into five groups of non-vitrified ovaries, vitrified ovaries, ovaries that were encapsulated with concentrations of 0.5, 0.75 and 1% of alginate hydrogel. The morphological study was performed using hematoxylin and eosin staining. Expression levels of apoptosis-associated genes were quantified in each group by real-time RT-PCR. The one-way ANOVA and post hoc test were used to analyz
Background:The purpose of this study was to determine the effects of alginate hydrogel as a capsule to protect the ovary against possible detrimental effects of vitrification and warming on morphology and expression of apoptosis-related genes in the mouse ovary.Methods:In this experimental study, the ovaries from twenty-five female 8-week-old mice were divided into five groups of non-vitrified ovaries, vitrified ovaries, ovaries that were encapsulated with concentrations of 0.5, 0.75 and 1% of alginate hydrogel. The morphological study was performed using hematoxylin and eosin staining. Expression levels of apoptosis-associated genes were quantified in each group by real-time RT-PCR. The one-way ANOVA and post hoc test were used to analyze
Ovarian transplantation is used to restore fertility potential and ovarian function in women with premature ovarian failure and in patients with cancer, and it can be performed using fresh or cryopreserved tissue in an autograft, allograft, and xenograft manner at orthotropic or heterotopic sites (1-4).Ischemia and degeneration of follicles are important factors, which should be considered during the transplantation of ovarian tissue and it can cause a decline in ovarian reserve and fertility potential (5-8). The vascular condition of the graft site plays a critical role in the reduction of ischemia. Therefore, angiogenesis in transplanted tissue and growth of the ovarian follicles depends on the graft site (5, 6, 8). Vascular endothelial g
Objective: Autograft transplantation of vitrified cortical ovarian tissue is an acceptable clinical technique for fertility preservation in women. Xenograft transplantation into animal models could be useful for evaluating the safety of human vitrified ovarian tissue. This study targeted to evaluate impact of vitrification on expression of the genes associated with folliculogenesis after xenograft transplantation of human vitrified ovarian tissue to γ-irradiated mice. Materials and Methods: In this experimental study, ovarian biopsies were gathered from six transsexual persons. The cortical section of ovarian biopsies was separated and chopped into small pieces. These pieces were randomly divided into vitrified and non-vitrified groups. In
Objective: The aim of the present study was to evaluate the effects of lysophosphatidic acid (LPA) supplementation of human ovarian tissue culture media on tissue survival, follicular development and expression of apoptotic genes following xenotransplantation.Materials and Methods: In this experimental study, human ovarian tissue was collected from eight normal female to male transsexual individuals and cut into small fragments. These fragments were vitrified-warmed and cultured for 24 hours in the presence or absence of LPA, then xenografted into back muscles of γ-irradiated mice. Two weeks post-transplantation the morphology of the recovered tissues were evaluated by hematoxylin and eosin staining. The expression of genes related to apop
Objective: The aim of the present study was to evaluate the effects of lysophosphatidic acid (LPA) supplementation of human ovarian tissue culture media on tissue survival, follicular development and expression of apoptotic genes following xenotransplantation.Materials and Methods: In this experimental study, human ovarian tissue was collected from eight normal female to male transsexual individuals and cut into small fragments. These fragments were vitrified-warmed and cultured for 24 hours in the presence or absence of LPA, then xenografted into back muscles of γ-irradiated mice. Two weeks post-transplantation the morphology of the recovered tissues were evaluated by hematoxylin and eosin staining. The expression of genes related to apop
The benefits of combined cell/material therapy appear promising for myocardial infarction treatment. The safety of alginate, along with its excellent biocompatibility and biodegradability, has been extensively investigated for cardiac tissue engineering. Among graphene-based nanomaterials, reduced graphene oxide has been considered as a promising candidate for cardiac treatment due to its unique physicochemical properties. In this study, the reduced graphene oxide incorporation effect within alginate hydrogels was investigated for cardiac repair application. Reduced graphene oxide reinforced alginate properties, resulting in an increase in gel stiffness. The cytocompatibility of the hydrogels prepared with human bone marrow–derived mesenc
Background & objectives: Oxaliplatin is a third-generation of platinum drug which is the main therapeutic agent for the treatment of colorectal cancer. Oxaliplatin not only inhibits DNA replication but also transcription and also induces apoptosis or necrosis in cancer cells and rapidly dividing cell lines. Although it is widely used clinically, there is no enough information regarding its effect on ovarian structure. This study was designed to determine morphometric features of 30-and 60-day-old offspring ovaries using precise and unbiased stereological technique following administration of oxaliplatin during perinatal period in mice.Methods: In the present experimental study, 32 adult female mice were used for experimental (pre-pregnant,
In vitro maturation (IVM) of oocytes is widely used in assisted reproduction technologies whereas the obtained results are not efficient. This study was aimed to improve the in vitro oocyte maturation and its development by supplementation of culture media with sodium selenite (SS). Moreover, the effects of SS on the expression of oocytes apoptosis-related genes was assessed. In this study, male and female NMRI mice were used and their germinal vesicle (GV) oocytes were collected and cultured with SS (experimental group) and without SS (control group). Also collected metaphase II oocytes (MII) from fallopian tube considered as in vivo group. After in vitro culture, the oocytes were evaluated for nuclear maturation. Some of the MII oocytes w
Methods: In this experimental study, 32 female NMRI mature mice were randomly allocated into 4 groups. Animals in control group were received 0.2 ml saline intraperitoneally (IP) during 21 days of pre-pregnany, pregnancy and lactation periods. Animals in experimental groups including pre-pregnant, pregnant and lactation groups were received 3 mg/kg oxaliplatin trice a week IP during 21 days before mating, during pregnancy and lactation periods, respectively. At the 60th postnatal day, all the male offspring were euthanized and sperm samples were obtained. Analysis of sperm parameters including count, motility, vitality, maturation and DNA integrity was done.Results: Sperm count, motility and DNA integrity were significantly reduced in all t