Fa
  • Ph.D. (2008)

    Medical Molecular Genetics

    INSERMU770, Université Paris Diderot, Paris, France

  • M.Sc. (2004)

    Human Genetics

    Department of Medical Genetics, Tehran University of Medical Sciences, Tehran, Iran

  • B.Sc. (1996)

    Midwifery

    ِِDepartment of Midwifery, Shahid Beheshti University of Medical Sciences, Tehran, Iran

  • Cancer Genetics
  • Translational Genomics
  • Translational Genomics Lab

    Research field: Cancer Genetics

    Expert:

    Phone: 021-82884516

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Curriculum Vitae (CV)

Effect of Acetamiprid on spatial memory and hippocampal glutamatergic system

Mohsen Shamsi, Maliheh Soodi, Shirin Shahbazi, Ameneh Omidi
Journal PaperEnvironmental Science and Pollution Research , 2021 February 1, {Pages 09-Jan }

Abstract

Acetamiprid (ACE) is one of the widely used neonicotinoid insecticides. In mammals, in spite of the low-affinity nAChRs, neurotoxic effects following the Acetamiprid exposure have recently been reported, which suggests some concerns regarding the impacts on the nervous system of mammals. This study aims to investigate the effect of Acetamiprid on spatial memory and possible vulnerability of hippocampal glutamatergic system following the Acetamiprid exposure. 10, 20, and 40 mg/kg doses of Acetamiprid were administered to male rats by gavage once per day for 28 days. The spatial memory was examined with the Morris water maze apparatus. The amount of Acetamiprid in the serum and hippocampus was measured. In addition, glutamate level and change

Lysophosphatidic Acid Alters The Expression of Apoptosis Related Genes and miR-22 in Cultured and Autotransplanted Ovaries

M Dehghan, S Shahbazi, M Salehnia
Journal Paper , , {Pages }

Abstract

Comparison of OGG1 gene expression level in gastric adenocarcinoma and adjacent normal tissue

S Moosaie, Z Afshari, A Abbasi, M Ghojaie, A Farhadi, MF Heidari, ...
Journal Paper , , {Pages }

Abstract

Expression Profile of MiR-200 Family Members and Their Targets in Prostate Cancer

M Khorasani, S Shahbazi, M Abolhasani, H Shahrokh, R Mahdian
Journal Paper , , {Pages }

Abstract

Effect of Lysophosphatidic Acid on the Vascular Endothelial Growth Factor Expression in Autotransplanted Mouse Ovaries Encapsulated in Sodium Alginate

M Dehghan, S Shahbazi, M Salehnia
Journal Paper , , {Pages }

Abstract

Follicular development and the expression of BAX and vascular endothelial growth factor in transplanted ovaries in uni-and bilateral ovariectomized mice: An experimental study

M Dehghan, S Shahbazi, M Salehnia
Journal Paper , , {Pages }

Abstract

Prediction and in silico validation of MYH7 gene missense variants in the Iranian population: a bioinformatics analysis based on Iranome database

Shirin Shahbazi
Journal PaperEgyptian Journal of Medical Human Genetics , Volume 21 , Issue 1, 2020 December , {Pages 07-Jan }

Abstract

Identifying disease-causing genetic variants in a particular population improves the molecular diagnosis of genetic disorders. National genome databases provide valuable information on this matter. This study aimed to investigate the genomic variants of the MYH7 gene, related to the common heart disease, i.e., hereditary cardiomyopathy. MYH7 gene variants were extracted from the Iranome database and loaded into SPSS software. The filtration steps were performed based on the variant specification and with emphasis on identifying missense changes. Using predictive algorithms, different aspects of the changes such as allele frequency and functional defects were investigated. Our results showed that 41 (17.4%) coding variants were synonymous co

ADAR expression and copy number variation in patients with advanced gastric cancer

Javad Behroozi, Shirin Shahbazi, Mohammad Reza Bakhtiarizadeh, Habibollah Mahmoodzadeh
Journal PaperBMC gastroenterology , Volume 20 , 2020 December , {Pages 09-Jan }

Abstract

Background Gastric cancer (GC) is a world health problem and it is the third leading cause of cancer deaths worldwide. The current practice for prognosis assessment in GC is based on radiological and pathological criteria and they may not result in an accurate prognosis. The aim of this study is to evaluate expression and copy number variation of the ADAR gene in advanced GC and clarify its correlation with survival and histopathological characteristics. Methods Forty two patients with stage III and IV GC were included in this study. ADAR gene expression and copy number variation were measured by real-time PCR and Quantitative multiplex fluorescent-PCR, respectively. Survival analysis performed based on the Kaplan–Meier method and Man

Comprehensive Characterization of RNA Editing in Primary Gastric Adenocarcinoma through RNA-seq Data Analysis

S Shahbazi, J Behroozi, MR Bakhtiarizadeh, H Mahmoodzadeh
Journal Paper , 2020 April 23, {Pages }

Abstract

RNA editing is a post-transcriptional nucleotide modification in humans. Of the various types of RNA editing, the adenosine to inosine substitution is the most widespread in higher eukaryotes, which is mediated by ADAR family enzyme. Inosine is recognized by the biological machineries as guanosine, therefore, editing can potentially rendering substantial functional effects throughout the genome, depending on where it located. RNA editing could contribute to cancer by either exclusive editing of tumor suppressor/promoting genes or by introducing transcriptomic diversity to promote cancer progression. Here, we provided a comprehensive overview of the RNA editing sites in gastric adenocarcinoma and highlighted some of their possible contributi

Comprehensive Characterization of RNA Editing in Primary Gastric Adenocarcinoma through RNA-seq Data Analysis

Javad Behroozi, Shirin Shahbazi, Mohammad Reza Bakhtiarizadeh, Habibollah Mahmoodzadeh
Journal Paper , 2020 April 23, {Pages }

Abstract

RNA editing is a post-transcriptional nucleotide modi cation in humans. Of the various types of RNA editing, the adenosine to inosine substitution is the most widespread in higher eukaryotes, which is mediated by ADAR family enzyme. Inosine is recognized by the biological machineries as guanosine, therefore, editing can potentially rendering substantial functional effects throughout the genome, depending on where it located. RNA editing could contribute to cancer by either exclusive editing of tumor suppressor/promoting genes or by introducing transcriptomic diversity to promote cancer progression. Here, we provided a comprehensive overview of the RNA editing sites in gastric adenocarcinoma and highlighted some of their possible contributio

ADAR expression and copy number variation in patients with advanced gastric cancer

S Shahbazi, J Behroozi, MR Bakhtiarizadeh, H Mahmoodzadeh
Journal Paper , 2020 January 27, {Pages }

Abstract

Background Gastric cancer (GC) is a world health problem and it is the third leading cause of cancer deaths worldwide. The current practice for prognosis assessment in GC is based on radiological and pathological criteria and they may not result in an accurate prognosis. The aim of this study is to evaluate expression and copy number variation of the ADAR gene in advanced GC and clarify its correlation with survival and histopathological characteristics. Methods Forty two patients with stage III and IV GC were included in this study. ADAR gene expression and copy number variation were measured by real-time PCR and Quantitative multiplex fluorescent-PCR, respectively. Survival analysis performed based on the Kaplan–Meier method and Mantel

Hematological indices in prolonged menstruation: New roles for blood groups and coagulation factors

Shirin Shahbazi
Journal PaperArchives of Medical Laboratory Sciences , Volume 4 , Issue 4, 2020 March 21, {Pages }

Abstract

Background: Menorrhagia is the most common type of abnormal uterine bleeding, caused by disruption of hormonal regulation, uterine function or blood clotting. Developing an effective diagnostic strategy will improve patient's quality of life and management. Here we investigated the links between hematological characteristics and prolonged menstruation to estimate the importance of the first line coagulation screening tests in young women.Materials and Methods: In a case-control design and under supervision of a specialist, 43 cases and 104 age matched controls were selected. Menstrual characteristics were evaluated by a standardized questionnaire distributed to high school and university young students. Hematological indices including first

The Effect of endurance training on expression of miR-21 and its downstream in breast cancer bearing mice

Sadegh Amani-Shalamzari, Hamid Agha Alinejad, Shirin Shahbazi, Shaban Alizadeh
Journal PaperJournal of Basic Research in Medical Sciences , Volume 7 , Issue 2, 2020 March 10, {Pages 47-57 }

Abstract

Materials and methods: After orientation to the environment, breast cancer cells, MC4-L2 were injected to mice and they randomly were categorized into two groups, control (n= 10) and training (n= 10) groups. Training group performed progressive endurance training 5 days per week for 6 weeks and control group did not perform any exercise. Tumor volume was measured by a digital caliper every week. Finally, the mice were sacrificed; tumor tissue was removed and immediately frozen and kept in-70 C. RNA extraction and cDNA synthesis were carried out by trizol reagent and specific kits and level of genes were measured by quantitative real-time PCR.Results: Endurance training decreased significantly expression of miR-21, STAT3 and Bcl2 (P< 0.05).

Detection of Paternal IVS-II-1 (G>A) (HBB: c.315+1G>A) Mutation in Cell-Free Fetal DNA Using COLD-PCR assay

Mohamad M Mortazavipour, Shirin Shahbazi, Reza Mahdian
Journal PaperHemoglobin , Volume 44 , Issue 3, 2020 May 3, {Pages 168-173 }

Abstract

Standardization of noninvasive prenatal diagnosis (PND) method that can identify common mutations in the population is of great value. The purpose of this study was to find the paternal HBB gene IVS-II-1 (G>A) (HBB: c.315+1G>A) mutation in maternal plasma cell-free DNA using the co-amplification at lower denaturation temperature-polymerase chain reaction (COLD-PCR) method. We designed simulated circulating free DNA (cfDNA) in maternal plasma to optimize the COLD-PCR assay. Peripheral blood samples were collected from normal and IVS-II-1 heterozygous individuals as well as five heterozygous pregnant women whose husbands were carriers of IVS-II-1. The cfDNA was extracted from the plasma and subjected to optimized COLD-PCR followed by Sanger s

Investigation of the SPAG5 gene expression and amplification related to the NuMA mRNA levels in breast ductal carcinoma

Zeinab Mohamadalizadeh-Hanjani, Shirin Shahbazi, Loabat Geranpayeh
Journal PaperWorld Journal of Surgical Oncology , Volume 18 , Issue 1, 2020 December , {Pages 10-Jan }

Abstract

The cell proliferative markers are very important in breast cancer. Since SPAG5 and NuMA proteins play a significant role in the mitosis regulatory network and cell division, we aimed to study their mRNA levels as well as SPAG5 gene amplification correlated to clinicopathological status in ductal carcinoma of the breast. SPAG5 and NuMA gene expressions were investigated in 40 breast cancer tissues and normal adjacent tissues via real-time PCR. PUM1 was selected as the reference gene. QMF PCR method was applied to study SPAG5 gene amplification and AGBL2, BOD1L, and POR were designated as internal control genes. Gene amplification was determined by calculating a dosage quotient for each DNA fragment. Increased SPAG5 mRNA expression was detec

Differential genes expression analysis of invasive aspergillosis: a bioinformatics study based on mRNA/microRNA

Maryam Hosseinipour, Shirin Shahbazi, Shahla Roudbar-Mohammadi, Maryam Khorasani, Majid Marjani
Journal PaperMolecular Biology Research Communications , Volume 9 , Issue 4, 2020 December 1, {Pages 173-180 }

Abstract

Invasive aspergillosis is a severe opportunistic infection with high mortality in immunocompromised patients. Recently, the roles of microRNAs have been taken into consideration in the immune system and inflammatory responses. Using bioinformatics approaches, we aimed to study the microRNAs related to invasive aspergillosis to understand the molecular pathways involved in the disease pathogenesis. Data were extracted from the gene expression omnibus (GEO) database. We proposed 3 differentially expressed genes; S100B, TDRD9 and TMTC1 related to pathogenesis of invasive aspergillosis. Using miRWalk 2.0 predictive tool, microRNAs that targeted the selected genes were identified. The roles of microRNAs were investigated by microRNA target predi

Genome-Wide Characterization of RNA Editing Sites in Primary Gastric Adenocarcinoma through RNA-seq Data Analysis

Javad Behroozi, Shirin Shahbazi, Mohammad Reza Bakhtiarizadeh, Habibollah Mahmoodzadeh
Journal PaperInternational Journal of Genomics , Volume 2020 , 2020 January 1, {Pages }

Abstract

RNA editing is a posttranscriptional nucleotide modification in humans. Of the various types of RNA editing, the adenosine to inosine substitution is the most widespread in higher eukaryotes, which is mediated by the ADAR family enzymes. Inosine is recognized by the biological machinery as guanosine; therefore, editing could have substantial functional effects throughout the genome. RNA editing could contribute to cancer either by exclusive editing of tumor suppressor/promoting genes or by introducing transcriptomic diversity to promote cancer progression. Here, we provided a comprehensive overview of the RNA editing sites in gastric adenocarcinoma and highlighted some of their possible contributions to gastric cancer. RNA-seq data correspo

Factor VII Gene Defects: Review of Functional Studies and Their Clinical Implications

Shirin Shahbazi, Reza Mahdian
Journal PaperIranian biomedical journal , Volume 23 , Issue 3, 2019 May , {Pages 165 }

Abstract

Coagulation factors belong to a family of plasma glycosylated proteins that should be activated for appropriate blood coagulation. Congenital deficiencies of these factors cause inheritable hemorrhagic diseases. Factor VII (FVII) deficiency is a rare bleeding disorder with variable clinical symptoms. Various mutations have been identified throughout the F7 gene and can affect all the protein domains. The results of previous experiments have partly revealed the correlation between genotype and phenotype in patients with FVII deficiency. Nevertheless, each particular variant may affect the coagulative function of FVII, mainly via altering its expression level, extra-cellular secretion, tissue factor binding affinity, or proteolytic activity.

The Effects of High Intensity Interval Training on Mir-23a Expression and Related Factors Involved in Muscular Atrophy of Aged Rats

Seyed Morteza Tayebi, Marefat Siahkouhian, Mohammad Keshavarz, Reza Mahdian, Mahdieh Mollanouri Shamsi, Shirin Shahbazi
Journal PaperInternational Journal of Applied Exercise Physiology , Volume 8 , Issue 1, 2019 January 22, {Pages 170-176 }

Abstract

Among the epigenetical factors involved in muscular atrophy, Mir-23a is subjected to change due to an alternation in intercellular calcium levels. The aim of this study is to investigate the chronic effect of high intensity interval training (HIIT) on Mir-23a expression and some other related factors, interpreting in Plantaris muscle atrophy of aged rats. Twenty-eight aged and young rats were divided into 4 different groups including exercise and control. The training protocol included 6 weeks of HIIT. Animals were sacrificed 48 hours after the last training session, and Plantaris muscle was removed. In order to measure Mir-23a, Rcan-1mRNA, Murf-1mRNA and Atrogin-1mRNA we used Real-time PCR technique. The results showed that aging significa

Q192R variant in paraoxonase 1 gene confers susceptibility to leiomyoma

Shirin Shahbazi, Soghra Zarei, Mahnaz Torfeh, Neda Fatahi
Journal Paper , 2019 June 27, {Pages }

Abstract

Objective: Paraoxonase 1 (PON1) plays a defensive role against oxidative stress by destroying oxidized lipids. Q192R single nucleotide polymorphism of PON1 gene alters the enzyme's activity. Several investigations reported a link between Q192R and an increased risk of developing tumors including uterine leiomyomas. We assessed the antioxidant effects of Q192R on myoma which fluctuate in frequency between populations. Study Design: The cohort consisted of 68 unrelated uterine leiomyoma patients and 93 healthy controls that were randomly selected from women with no ultrasonographic evidence of myoma. Materials and Methods: Genotyping was performed using tetra-primer amplification refractory mutation system-polymerase chain reaction. Chi-squar

Current Teaching

  • MS.c.

    Human Biochemical Genetics

  • MS.c.

    Molecular Genetics

  • MS.c.

    Molecular Genetics

  • MS.c.

    Population Genetics and Epidemiology

  • Ph.D.

    Advanced Molecular Genetics

  • Ph.D.

    Advanced Molecular Genetics

Teaching History

  • MS.c.

    Cancer Genetics

  • MS.c.

    Genetic Engineering

  • Ph.D.

    Genetic treatment of diseases

  • MS.c.

    Somatic and Cytoplasmic Genetics

  • Ph.D.

    Special hospital training

  • 2021
    Bazgir, Afsaneh
    Investigating the expression levels of ADARs genes and their correlation to the expression and RNA editing of the RhoA gene and the expression of RhoA-IT1 long non-coding RNA in invasive ductal carcinoma of the breast

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