Fa
  • Ph.D. (2000)

    Parasitology

    , Tarbiat Modares University,

  • M.Sc. (1994)

    Parasitology

    , Tarbiat Modares University,

  • New Generation Vaccines (DNA vaccines)
  • New methods for treating parasitic diseases and employing nanoparticles and examining the expression of genes involved in the prevention and treatment of parasitic diseases
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Curriculum Vitae (CV)

Immunogenicity of in-silico designed multi-epitope DNA vaccine encoding SAG1, SAG3 and SAG5 of Toxoplasma gondii adjuvanted with CpG-ODN against acute toxoplasmosis in BALB/c mice

Masoumeh Khodadadi, Fatemeh Ghaffarifar, Abdolhosein Dalimi, Ehsan Ahmadpour
Journal PaperActa Tropica , Volume 216 , 2021 April 1, {Pages 105836 }

Abstract

The causative agent of toxoplasmosis, Toxoplasma gondii (T. gondii), is able to influence the health of humans and other vertebrates. Toxoplasma may cause severe illness in the fetus and immunocompromised individuals. The high incidence and intense damages of Toxoplasma infection clearly shows the need to achieve the safe and suitable vaccine. In this study, an immunoinformatics approach was employed to design a multi-epitope DNA vaccine encoding the T. gondii SAG1, SAG3 and SAG5. The bioinformatic outputs supported the immunogenic and non-allergic natures of multi-epitope vaccine. Thereafter, the protective efficacy of the vaccine was evaluated with/without CpG-ODN adjuvant in a laboratory animal model. BALB/c mice were immunized subcutane

Immunoinformatic analysis of immunogenic B-and T-cell epitopes of MIC4 protein to designing a vaccine candidate against Toxoplasma gondii through an in-silico approach

Ali Dalir Ghaffari, Abdolhossein Dalimi, Fatemeh Ghaffarifar, Majid Pirestani, Hamidreza Majidiani
Journal PaperClinical and experimental vaccine research , Volume 10 , Issue 1, 2021 January , {Pages 59 }

Abstract

PurposeToxoplasmosis, transmitted by Toxoplasma gondii, is a worldwide parasitic disease that affects approximately one-third of the world's inhabitants. Today, there are no appropriate drugs to deter tissue cysts from developing in infected hosts. So, developing an effective vaccine would be valuable to avoid from toxoplasmosis. Considering the role of microneme antigens such as microneme protein 4 (MIC4) in T. gondii pathogenesis, it can be used as potential candidates for vaccine against T. gondii.Materials and MethodsIn this study several bioinformatics methods were used to assess the different aspects of MIC4 protein such as secondary and tertiary structure, physicochemical characteristics, the transmembrane domains, subcellular locali

Multi-epitope vaccine expressed in Leishmania tarentolae confers protective immunity to Toxoplasma gondii in BALB/c mice

Hamidreza Majidiani, Abdolhossein Dalimi, Fatemeh Ghaffarifar, Majid Pirestani
Journal PaperMicrobial Pathogenesis , 2021 April 29, {Pages 104925 }

Abstract

Current study deals with a novel multi-epitope vaccine designed in silico and its confirmation experiments for potential efficacy in BALB/c mice. Major histocompatibility complex (MHC)-binding and B-cell binding epitopes of five Toxoplasma antigens (SAG1, ROP16, GRA12, MIC4 and M2AP) were predicted. Selected epitopes were fused together using SAPGTP linker, and antigenicity, allergenicity, physico-chemical features, secondary and tertiary structures and validations were all performed via bioinformatics servers. Then, vaccine construct was cloned into pLEXSY-neo 2.1 vector. After Leishmania tarentolae transfection, live recombinant and wild parasites were subcutaneously injected into 6–8 week female BALB/c mice and immune responses were me

Silybum marianum ethanolic extract: in vitro effects on protoscolices of Echinococcus granulosus G1 strain with emphasis on other Iranian medicinal plants

A Taghipour, F Ghaffarifar, J Horton, A Dalimi, Z Sharifi
Journal Paper , , {Pages }

Abstract

Therapeutic and Preventive Effects of Morphine Against Leishmania Major and Evaluation the Expression of TLRs and Cytokines in Infected Macrophages in Vitro and in BALB/c Mice

P Ebrahimisadr, F Ghaffarifar, J Jabari, J Horton, Z Sharifi, A Dalimi, ...
Journal Paper , , {Pages }

Abstract

Synergistic Anti-Leishmanial Activities of Morphine and Imiquimod on Leishmania infantum (MCAN/ES/98/LIM-877)

F Ghaffarifar, M Foroutan, S Molaei, E Moradi-Asl
Journal Paper , , {Pages }

Abstract

Efficacy of green synthesized silver nanoparticles via ginger rhizome extract against Leishmania major in vitro

M Mohammadi, L Zaki, A KarimiPourSaryazdi, P Tavakoli, A Tavajjohi, ...
Journal Paper , , {Pages }

Abstract

Toxoplasma gondii in Slaughtered Sheep in High-and Low-Humidity Regions in the South of Iran: Molecular Prevalence and Genotype Identification

SZ Khademi, F Ghaffarifar, A Dalimi, MS Dayer, A Abdoli
Journal Paper , , {Pages }

Abstract

Synthesis of Tellurium Oxide (TeO2) Nanorods and Nanoflakes and Evaluation of Its Efficacy Against Leishmania major In Vitro and In Vivo

P Tavakoli, F Ghaffarifar, H Delavari, A KarimiPourSaryazdi, MS Dayer, ...
Journal Paper , , {Pages }

Abstract

Evaluation of Anti-amoebic Activity of Spring Leaf Extract of Artemisia Aucheri on Acanthamoeba (T4 strain) In vitro

A KarimiPourSaryazdi, Y KarimiPourSaryazdi, F Ghaffarifar, M Ahmadi, ...
Journal Paper , , {Pages }

Abstract

In Vitro and In Vivo Anti-parasitic Activity of Artemisinin Combined With Glucantime and Shark Cartilage Extract on Iranian Strain of Leishmania major (MRHO/IR/75/ER)

F Ghaffarifar, S Molaei, Z Mohammad Hassan, MS Dayer, A Dalimi, ...
Journal Paper , , {Pages }

Abstract

In Vitro and In Vivo Anti-parasitic Activity of Artemisinin Combined With Glucantime and Shark Cartilage Extract on Iranian Strain of Leishmania major (MRHO/IR/75/ER)

F Ghaffarifar, S Molaei, ZM Hassan, MS Dayer, A Dalimi, V Nasiri, ...
Journal Paper , , {Pages }

Abstract

Evaluation of the effects of Rumex obtusifolius seed and leaf extracts against Acanthamoeba: An in vitro study

T Nayeri, F Bineshian, F Khoshzaban, AD Asl, F Ghaffarifar
Journal Paper , , {Pages }

Abstract

Comparison of the Effects of Sambucus ebulus Leaf and Fruit Extracts on Leishmania major In Vitro

S Kadkhodamasoum, F Bineshian, A KarimiPour, P Tavakoli, M Foroutan, ...
Journal Paper , , {Pages }

Abstract

A study on the in vitro and in vivo effects of aqueous and alcoholic extracts of Salvia mirzayanii on Leishmania major.

F Hasanzadeh, F Ghaffarifar, S Mehdizadeh, MS Dayer
Journal Paper , , {Pages }

Abstract

Enhancing immune responses by a novel multi-epitope ROP8 DNA vaccine plus interleukin-12 plasmid as a genetic adjuvant against acute Toxoplasma gondii infection in BALB/c mice

Masoud Foroutan, Mohammad Barati, Fatemeh Ghaffarifar
Journal PaperMicrobial Pathogenesis , Volume 147 , 2020 October 1, {Pages 104435 }

Abstract

BackgroundToxoplasmosis is a widespread zoonotic infection, caused by an obligate intracellular protozoan. The infection is often asymptomatic in immunocompetent individuals, although in persons with impaired immune system may lead to severe and progressive complications. Constant attempts of scientists have made valuable findings in the development of Toxoplasma gondii (T. gondii) candidate vaccines. However, an effective vaccine has not been successfully developed yet. In the current study, we tested the co-delivery of a novel multi-epitope pcROP8 DNA vaccine with a plasmid encoding IL-12 (pcIL-12) to assess the immune responses in BALB/c mice against acute T. gondii infection.MethodsBALB/c mice were immunized on days 0, 21, and 42. The i

In Vitro Effect of Some Medicinal Plants on Leishmania major Strain MRHO/IR/75/ER

Mojtaba Raeisi, Kamal Mirkarimi, Behrooz Jannat, Bahman Rahimi Esboei, Abdol Sattar Pagheh, Zahra Mehrbakhsh, Fatemeh Ghaffarifar, Oghlniaz Jorjani, Masoud Foroutan
Journal PaperMedical Laboratory Journal , Volume 14 , Issue 4, 2020 July 10, {Pages 46-52 }

Abstract

Methods: The essential oils were prepared from freshly dried and powdered plants with steam-distilled water. Iranian strain of Leishmania promastigotes was cultured in RPMI medium and the inhibitory effects of different concentrations (25, 32, 62.5, 125, 250, 500 and 1000 μg/ml) of the essential oils were investigated at 24, 48 and 72 hours. The number of live parasites before and after treatment with the essential oils was counted by trypan blue 10% staining and using neobar lam.Results: The essential oils significantly decreased the number of promastigotes in a dose-dependent manner (P< 0.05). However, the inhibitory effects of F. vulgare and R. officinalis essential oils were more profound compared to other essential oils. Moreover, con

Evaluation of Asymptomatic Malaria Status in Eastern of Afghan-istan Using High Resolution Melting Analysis

Sayed Hussain MOSAWI, Abdolhossein DALIMI, SAFI Najibullah, Fatemeh GHAFFARIFAR, Javid SADRAEI
Journal PaperIranian Journal of Parasitology , Volume 15 , Issue 2, 2020 June 7, {Pages 177-186 }

Abstract

Background: Malaria is threatening more than half of Afghanistan population. Asymptomatic malaria is notable problem against malaria controlling strategies. In this study we evaluated the asymptomatic malaria status in Nangarhar Province, Afghanistan in 2017. Methods: Overall, 296 finger blood samples were taken on DNA Banking Cards and microscopic slides from asymptomatic individuals in Jalalabad city. We used a novel post real time PCR high resolution melting analysis beside microscopy and semi-nested multiplex PCR to evaluate status of asymptomatic malaria in this city. Results: The prevalence of asymptomatic malaria in Jalalabad city was determined 1.7%(5/296), 7.43%(22/296) and 7.78%(26/296) by microscopy, Seminested multiplex PCR and

In-vitro and in-vivo comparative effects of the spring and autumn-harvested Artemisia aucheri Bioss extracts on Leishmania major

Amir Karimipoursaryazdi, Fatemeh Ghaffarifar, Abdolhossein Dalimi, Mohammad Saaid Dayer
Journal PaperJournal of Ethnopharmacology , 2020 April 25, {Pages 112910 }

Abstract

Ethnopharmacological relevanceArtemisia aucheri Bioss contains flavonoid, coumarin and santonin with antioxidant, antimicrobial and antileishmanial effects. The current study was aimed to comparatively evaluate the effects of spring and autumn extracts of A. aucheri Bioss on Leishmania major both in-vitro and in-vivo conditions.MethodsHPLC analysis was used to evaluate the percentages of compounds in spring and autumn extracts of A. aucheri. For in-vitro assay, the effect of different concentrations of spring and autumn extracts of A. aucheri was tested on L. major promastigotes and amastigotes. MTT and flow cytometry methods were used to evaluate the cytotoxicity and probable apoptosis of A. aucheri extracts on L. major promastigotes. On t

Evaluation of the effects of Rumex obtusifolius seed and leaf extracts against Acanthamoeba: An in vitro study.

Tooran Nayeri, Farahnaz Bineshian, Fariba Khoshzaban, Abdolhossein Dalimi Asl, Fatemeh Ghaffarifar
Journal PaperInfectious disorders drug targets , 2020 April 22, {Pages }

Abstract

BACKGROUND: Acanthamoebiasis treatment is a major and challenging problem due to the presence of resistant cyst form. Many herbal extracts and their derivatives have been used against trophozoites and cysts of Acanthamoeba, but no effective therapeutic agent has yet been discovered. Therefore, the present study aimed to evaluate the effect of Rumex obtusifolius (R. obtusifolius) extracts against a clinical strain of Acanthamoeba genotype T4 in vitro. METHODS: In this experimental study, after genotyping the clinical isolate, the hydroalcohlic extracts of R. obtusifolius seeds and leaves were prepared. Different concentrations (1.25, 2.5, 5 and 10 mg/ml) of extracts were tested in triplicate (24, 48 and 72h) on trophozoites and cysts of Acan

Current Teaching

  • MS.c.

    Helminthology II

  • MS.c.

    Helminthology II

  • Ph.D.

    --

  • Ph.D.

    --

Teaching History

  • MS.c.

    --

  • MS.c.

    General Leishmaniasis

  • MS.c.

    Helminthology I

  • MS.c.

    Internship

  • 2020
    Karimipour saryazdi, Amir
    Designing of DNA encoding multi-epitope fragments of ROP21 and ROP29 genes as candidate vaccine and it\'s immunogenic evaluation against acute and chronic toxoplasmosis in BALB/c mice

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