Fa
  • Ph.D. (2013)

    biochemistry

    , Tarbiat Modares University,

  • M.Sc. (2007)

    Biology - Biochemistry

    , Al-Zahra University / QA /,

  • B.Sc. (2004)

    Biology - Cellular and molecular sciences

    , University of Tehran, Iran

  • Engineering of therapeutic proteins production in HEK, CHO and Ecoli
  • Studying different signaling pathways in cancer cells with the aim of diagnosis, drug design and delivery

    Contact

    Curriculum Vitae (CV)

    Cancer‐associated‐platelet‐inspired nanomedicines for cancer therapy

    Marzieh Geranpayehvaghei, Bahareh Dabirmanesh, Mohammad Khaledi, Mona Atabakhshi‐Kashi, Chao Gao, Mohammad Taleb, Yinlong Zhang, Khosro Khajeh, Guangjun Nie
    Journal Paper , 2021 February 3, {Pages e1702 }

    Abstract

    Platelets, with hemostasis and thrombosis activities, are one of the key components in the blood circulation. As a guard, they rapidly respond to any abnormal blood vessel injury signal and release their granules' contents, which induce their adhesion and aggregation on wound site for hemostasis. Recently, increasing evidence has indicated that platelets are critically involved in the growth and metastasis of cancer cells by releasing a variety of cytokines and chemokines to stimulate cancer cell proliferation and various angiogenic regulators to accelerate tumor angiogenesis. Platelets also secrete active transforming growth factor beta (TGF‐β) to promote the epithelial–mesenchymal transition of cancer cells and their extravasation f

    Enhanced Solubility and One-Step Purification of Functional Dimeric Carboxypeptidase G2

    Atefeh Khodakarami, Bahareh Dabirmanesh, Sedigheh Asad, Mohammad Khaledi
    Journal PaperBiochemistry (Moscow) , Volume 86 , Issue 2, 2021 February , {Pages 190-196 }

    Abstract

    Carboxypeptidase G2 is a bacterial enzyme that catalyzes methotrexate conversion to its inactive forms which are then eliminated via a non-renal pathway in patients with renal disorders during a high-dose methotrexate administration. Due to the increasing demand of this enzyme, it was of interest to simplify its production process. For this reason, we developed a method for production and one-step purification of this enzyme using an intein-mediated system with a chitin-binding affinity tag. The carboxypeptidase G2 gene from Pseudomonas RS16 was optimized, synthesized, cloned into the pTXB1 expression vector and finally transformed into Escherichia coli BL21 (DE3) cells. The optimal condition for the enzyme soluble expression was achieved i

    Screening and Identification of Herbal Urease Inhibitors Using Surface Plasmon Resonance Biosensor

    Mahmood Biglar, Hafezeh Salehabadi, Safoura Jabbari, Bahareh Dabirmanesh, Khosro Khajeh, Faraz Mojab, Massoud Amanlou*
    Journal PaperResearch Journal of Pharmacognosy , Volume 8 , Issue 2, 2021 April 1, {Pages 51-62 }

    Abstract

    Background and objectives: Urease, that catalyzes the hydrolysis of urea, has received substantial attention for its impact on living organisms’ health and human life quality. Urease inhibitors play important role in management of different diseases including gastritis and other gastrointestinal disorders. In the present study, a new surface plasmon resonance-based biosensor was designed to discover new urease inhibitors. Methods: The biosensor surface was prepared by the covalent immobilization of urease on carboxymethyldextran hydrogel (CMD 500D) via its primary amine groups. Results: The biosensor combined with an orthogonal enzyme inhibition assay was utilized for screening of 40 traditional medicinal plant extracts against Jack-bean

    Proteomic profiling of the rat hippocampus from the kindling and pilocarpine models of epilepsy: potential targets in calcium regulatory network

    Leila Sadeghi, Albert Anatolyevich Rizvanov, Bahareh Dabirmanesh, Ilnur Ildusovich Salafutdinov, Mohammad Sayyah, Amir Shojaei, Javad Zahiri, Javad Mirnajafi-Zadeh, Babak Khorsand, Khosro Khajeh, Yaghoub Fathollahi
    Journal PaperScientific reports , Volume 11 , Issue 1, 2021 April 15, {Pages 12-Jan }

    Abstract

    Herein proteomic profiling of the rat hippocampus from the kindling and pilocarpine models of epilepsy was performed to achieve new potential targets for treating epileptic seizures. A total of 144 differently expressed proteins in both left and right hippocampi by two-dimensional electrophoresis coupled to matrix-assisted laser desorption-mass spectrometry were identified across the rat models of epilepsy. Based on network analysis, the majority of differentially expressed proteins were associated with Ca 2+ homeostasis. Changes in ADP-ribosyl cyclase (ADPRC), lysophosphatidic acid receptor 3 (LPAR3), calreticulin, ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1), synaptosomal nerve-associated protein 25 (SNAP 25) and transgelin 3 protein

    CD38 and MGluR1 as possible signaling molecules involved in epileptogenesis: a potential role for NAD+ homeostasis

    Shima Khodaverdian, Elahe Dashtban-Moghadam, Bahareh Dabirmanesh, Javad Mirnajafi-Zadeh, Mohammad Taleb, Khosro Khajeh, Yaghoub Fathollahi
    Journal PaperBrain Research , 2021 April 28, {Pages 147509 }

    Abstract

    In spite of long-term intensive scientific research efforts, there are still many issues concerning the mechanisms of epileptogenesis and epilepsy to be resolved. Temporal lobe, in particular hippocampus, is vulnerable to epileptogenic process. Herein, electrical kindling model of temporal lobe were analyzed using proteomic approach. A dramatic decrease in nicotinamide adenine dinucleotide (NAD+) level was exhibited during the kindling procedure in hippocampus. After stage 3, high CD38 expression was detected by qPCR, nano-liquid chromatography-tandem mass spectrometry (nano-LC-MS/MS) and western blot analysis. An increase in expression of CD38/NADase activity was observed during the kindling procedure in hippocampus that represent it as on

    Extracting Potential New Targets for Treatment of Adenoid Cystic Carcinoma Using Bioinformatic Methods

    TF Pordanjani, B Dabirmanesh, P Choopanian, M Mirzaie, S Mohebbi, ...
    Journal Paper , , {Pages }

    Abstract

    Characterization of Interaction of the MBP-Tagged MuRif1-C-Terminal Domain with G-Quadruplex DNA by SPR

    H Ghadiri, S Alavi, B Dabirmanesh, K Khajeh
    Journal Paper , , {Pages }

    Abstract

    ИЗУЧЕНИЕ ВЗАИМОДЕЙСТВИЯ С-КОНЦЕВОГО ДОМЕНА БЕЛКА MuRif1 МЕЧЕННОГО MBP С G-КВАДРУПЛЕКСАМИ ДНК С ПОМОЩЬЮ …

    H Ghadiri, S Alavi, B Dabirmanesh, K Khajeh
    Journal Paper , , {Pages }

    Abstract

    Catalytic parameters and thermal stability of chondroitinase ABCI on red porous silicon nanoparticles

    Sara Daneshjou, Bahareh Dabirmanesh, Fereshteh Rahimi, Safoura Jabbari, Khosro Khajeh
    Journal PaperJournal of Biotechnology , Volume 324 , 2020 December 20, {Pages 83-90 }

    Abstract

    The bacterial enzyme chondroitinase ABC, which digests extracellular chondroitin sulfate proteoglycans, has been shown to enhance axonal regeneration. However, the utilization of this enzyme as therapeutics is notably restricted due to its thermal instability. Therefore, red luminescent porous silicon that hold promise for potential applications in biological/medical imaging was used as a carrying matrix for chondroitinase with the aim of enhancing its stability. Porous Si nanoparticles were prepared by electrochemical etching of silicon wafers in ethanolic HF solution. The size of nanoparticles (210 nm) and the mean pore diameter (8 −20 nm) were determined using dynamic light scattering and scanning electron microscopy. Purified chon

    A multifunctional fusion peptide for tethering to hydroxyapatite and selective capture of bone morphogenetic protein from extracellular milieu

    Mina Bahri, Sadegh Hasannia, Bahareh Dabirmanesh, Alireza Moshaverinia, Homayoun H Zadeh
    Journal PaperJournal of Biomedical Materials Research Part A , Volume 108 , Issue 7, 2020 July , {Pages 1459-1466 }

    Abstract

    Purpose The present study sought to design a multi‐functional fusion peptide with hydroxyapatite (HA) binding domain (HABD) and heparin binding domain (HBD). Methods The 74 amino acid fusion peptide contained N‐terminus of the fibrinogen β chain (β 15–66), double G4S‐linker and 12 residues with HA affinity. This construct was designed, synthesized and cloned into pET21a(+) vector and expressed in E. coli. Results HABD facilitated purification of the fusion peptide by HA affinity chromatography. Kinetic peptide binding and release on HA scaffold showed sustained release of peptide for up to 16 days. Competitive ELISA and intrinsic fluorescence assays were applied to determine HBD affinity to bone morphogenetic protein‐2 (

    Enhancing myelin repair in experimental model of multiple sclerosis using immobilized chondroitinase ABC I on porous silicon nanoparticles

    Safoura Rezaei, Bahareh Dabirmanesh, Leila Zare, Abolfazl Golestani, Mohammad Javan, Khosro Khajeh
    Journal PaperInternational Journal of Biological Macromolecules , Volume 146 , 2020 March 1, {Pages 162-170 }

    Abstract

    Removal of chondroitin sulfate proteoglycans (CSPGs) with chondroitinase ABC I (ChABC) facilitates axonal plasticity, axonal regeneration and remyelination, following injury to the central nervous system (CNS). However, the ChABC rapidly undergoes thermal inactivity and needs to be injected repeatedly. Here this limitation was overcame by immobilizing the ChABC on porous silicon (PSi) nanoparticles (ChABC@PSi). The efficacy of immobilized ChABC on CSPGs level and the demyelination insult was assessed in mice corpora callosa demyelinated by 6?weeks cuprizone (CPZ) feeding. ChABC@PSi was able to reduce the amount of CSPGs two weeks after animals treatment. CSPGs digestion by ChABC@PSi reduced the extent of demyelinated area as well as the ast

    Anti-amyloidogenic effect of artemin on α-synuclein

    Narges Marvastizadeh, Bahareh Dabirmanesh, Reza H Sajedi, Khosro Khajeh
    Journal PaperBiological Chemistry , Volume 1 , Issue ahead-of-print, 2020 July 16, {Pages }

    Abstract

    α-Synuclein fibrillation is now regarded as a major pathogenic process in Parkinson’s disease and its proteinaceous deposits are also detected in other neurological disorders including Alzheimer's disease. Therefore anti-amyloidegenic compounds may delay or prevent the progression of synucleinopathies disease. Molecular chaperones are group of proteins which mediate correct folding of proteins by preventing unsuitable interactions which may lead to aggregation. The objective of this study was to investigate the anti-amyloidogenic effect of molecular chaperone artemin on α-synuclein. As the concentration of artemin was increased up to 4?μg/ml, a decrease in fibril formation of α-synuclein was observed using thioflavin T (ThT) fluoresce

    Molecular Dynamics Study of the Human Beta-defensins 2 and 3 Chimeric Peptides with the Cell Membrane Model of Pseudomonas aeruginosa

    Mohammad Davoud Ghafari, Iraj Rasooli, Khosro Khajeh, Bahareh Dabirmanesh, Parviz Owlia
    Journal PaperInternational Journal of Peptide Research and Therapeutics , 2020 January 1, {Pages 18-Jan }

    Abstract

    Pseudomonas aeruginosa is a unique microorganism among the antibiotic-resistant microbial pathogens. Among the various therapeutic approaches, antimicrobial peptides are effective to combat this infection. The chimera C3 is a peptide derived from the human beta-defensins 2 and 3 that has previously displayed antibacterial activity against the Gram-negative and Gram-positive bacteria. In this research, the antibacterial activity and the effect of a new genetically designed chimera C3 (i.e. chimera C3-3) on the bacterial membrane was assessed by molecular dynamics (MD) simulation. To investigate the interactions of the peptide with the lipid bilayer model and their effects on each other, the characterizations e.g. Area Per Lipid (APL), densi

    Evaluation of changes expression of D1, mGluR1 and CD38 genes after electrical kindling and treatment with low-frequency stimulation in rat

    Khosro Khajeh, Javad Mirnajafi-Zadeh, Bahareh Dabirmanesh, Shima Khodaverdian
    Journal PaperModares Journal of Biotechnology , Volume 11 , Issue 1, 2020 March 10, {Pages 37-43 }

    Abstract

    Evaluation of changes expression of D1 mGluR1 and CD38 genes after electrical kindling and treatment with low-frequency stimulation in rat-Tarbiat Modares University Journals System-Modares Journal of Biotechnology

    A review of fibrin applications and it’s derivatives in wound healing and tissue engineering

    Shahriar Hasannia, Mina Bahri, Fatemeh Gashtasbi, Bahareh Dabirmanesh
    Journal Paper , Volume 11 , Issue 3, 2020 October 10, {Pages 0-0 }

    Abstract

    Fibrinogen is a major component of the coagulation cascade following tissue damage and rapidly forms an insoluble fibrin scaffold. Fibrin is a filamentous biopolymer that naturally forms from fibrinogen polymerization during blood clotting. After tissue damage and coagulation cascade initiation, soluble fibrinogen polymerization by thrombin enzyme begins and forms an insoluble fibrin network and blood clots with platelets. This fibrin network is crucial for the development of homeostasis after tissue damage. This biopolymer also plays a key role in wound healing as a temporary scaffold and due to its unique structural properties and physiological function, it is used in reconstructive medicine. Fibrin is able to absorb extracellular matrix

    G-quadruplex binding protein Rif1, a key regulator of replication timing

    Sana Alavi, Hamed Ghadiri, Bahareh Dabirmanesh, Kenji Moriyama, Khosro Khajeh, Hisao Masai
    Journal PaperThe Journal of Biochemistry , 2020 November 10, {Pages }

    Abstract

    DNA replication is spatially and temporally regulated during S phase to execute efficient and coordinated duplication of entire genome. Various epigenomic mechanisms operate to regulate the timing and locations of replication. Among them, Rif1 plays a major role to shape the “replication domains” that dictate which segments of the genome are replicated when and where in the nuclei. Rif1 achieves this task by generating higher-order chromatin architecture near nuclear membrane and by recruiting a protein phosphatase. Rif1 is a G4 binding protein, and G4 binding activity of Rif1 is essential for replication timing regulation in fission yeast. In this article, we first summarize strategies by which cells regulate their replication

    Cloning, expression and characterization of carboxypeptidase G2 enzyme from Pseudomonas sp. strain RS-16 in E. coli

    Reza Alizadeh, Nasrin Kardani, Atefeh Khodakarami, Khosro Khajeh, Bahareh Dabirmanesh
    Journal Paper , Volume 26 , Issue 10, 2020 January 1, {Pages 18-Aug }

    Abstract

    Background: Methotrexate is one of the most widely used chemotherapeutic agents that may cause kidney failure as a side effect. Carboxypeptidase G2 (Glucarpidase marketed under the brand name of Voraxaze) is a bacterial enzyme that can convert methotrexate to its inactive metabolites and provides an alternative non-renal pathway for methotrexate elimination in patients with renal dysfunction during high-dose methotrexate treatment. Methods: In this research, carboxypeptidase G2 was synthetized in pUC 57 vector. Then it was subcloned into pET28a between NdeI and XhoI restriction sites. Recombinant vector was transformed into E. coli BL21 and its expression was examined in various conditions.Results: The optimum expression of recombinant prot

    Evaluation of changes expression of D1, mGluR1 and CD38 genes after electrical kindling and treatment with low-frequency stimulation in rat

    K Khajeh, J Mirnajafi-Zadeh, B Dabirmanesh, S Khodaverdian
    Journal Paper , , {Pages }

    Abstract

    Evaluation of Changes Expression of D1, mGluR1 and CD38 Genes after Electrical Kindling and Treatment with Low-Frequency Stimulation in Rats

    S Ranjbar, K Kh, SJ Mirnajafi-Zadeh, B Dabirmanesh, K Sh
    Journal Paper , , {Pages }

    Abstract

    Cloning, expression and Characterization of Carboxypeptidase G2 Enzyme from E. coli

    Reza Aalizade, Nasrin Kardan, Atefeh Khodakarami, Khosro Khajeh, Bahareh Dabirmanesh
    Journal PaperRazi Journal of Medical Sciences , Volume 26 , Issue 10, 2019 December 10, {Pages 18-Aug }

    Abstract

    Background: Methotrexate is one of the most widely used chemotherapeutic agents that may cause kidney failure as a side effect. Carboxypeptidase G2 (Glucarpidase marketed under the brand name of Voraxaze) is a bacterial enzyme that can convert methotrexate to its inactive metabolites and provides an alternative non-renal pathway for methotrexate elimination in patients with renal dysfunction during high-dose methotrexate treatment.Methods: In this research, carboxypeptidase G2 was synthetized in pUC 57 vector. Then it was subcloned into pET28a between NdeI and XhoI restriction sites. Recombinant vector was transformed into E. coli BL21 and its expression was examined in various conditions.Results: The optimum expression of recombinant prote

    Current Teaching

    • MS.c.

      Methods in Biochemistry & Molecular Biology

    • MS.c.

      Methods in Biochemistry & Molecular Biology

    Teaching History

    • MS.c.

      Advanced Molecular Biology

    • Ph.D.

      Advanced Topics in Biochemistry

    • 2021
      Rivand, Helia
      Design, synthesis, expression and purification of Reteplase using SUMO-tag
    • 2019
      Poordad, Zahra
      Inhibition of c-Met biomarker function by a novel inhibitory scfv and its effect on the proliferation and migration of colorectal cancer cells

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