Department of Bacteriology (2010 - Present)
Medical Bacteriology
, Isfahan University of Medical Sciences, Isfahan, Iran
Medical Microbiology
, Tehran University of Medical Sciences,
Biology - Microbiology
, Al-Zahra University / QA /,
Research field: Bacterial Vaccines, Designing Nano Biosensors,Molecular epidemiology
Expert:
Phone: 02182884558
Address: Ground floor of the medical building , room No 7
Campylobacter jejuni (C. jejuni) is a leading cause of acute gastroenteritis in human worldwide. The aim of study was to assess the distribution of sialylated lipooligosaccharide (LOS) classes and capsular genotypes in C. jejuni isolated from Iranian children with gastroenteritis. Furthermore, the level of dnaK gene expression in C. jejuni strains with selected capsular genotypes and LOS classes was intended. Moreover, a comprehensive study of C. jejuni MLST-genotypes and inclusive comparison with peer sequences worldwide was intended. Twenty clinical C. jejuni strains were isolated from fecal specimens of 280 children aged 0–5 years, suspected of bacterial gastroenteritis, which admitted to 3 children hospitals from May to October, 201
Background: Cholera could cause severe watery diarrhea and even death, but in patients diagnosed with severe dehydration, treatment with an appropriate antimicrobial agent could decrease the volume of diarrhea and shorten the duration of V. cholerae shedding. Unfortunately, due to the high antibiotic’s consumption, antibiotic resistance in V. cholerae strains is progressively increasing worldwide. The present systematic review and meta-analysis study aimed to investigate the incidence of antibiotic resistance in V. cholerae strains worldwide.Methods: The incidence of antibiotic resistance in V. cholerae strains was assessed by conducting a systematic review and searching international databases including PubMed and Google Scholar for arti
Co-infection of intestinal parasitic infections (IPIs) and Campylobacter spp. are public health problem in both developing and developed countries. This study was conducted to determine prevalence of IPIs and Campylobacter spp. among children with gastrointestinal disorders in Tehran. In this descriptive cross-sectional survey, 283 fresh stool samples were collected from all individuals and examined by standard parasitological methods including direct slide smear, formalin-ether concentration, trichrome staining, modified Ziehl-Neelsen staining, and chromotrope 2R staining techniques were used for detection of intestinal protozoa and helminths. Furthermore, culture and multiplex-PCR were also used to identify the species of Campylobacter. D
Removal of bacterial contaminations from water using advanced technologies is one of the essential steps in improving human health. The present study’s aim was to develop high performance microfiltration membranes from polyacrylonitrile (PAN) for bacteria removal from drinking water. The characteristics and performance of membranes were tuned through exploring the variation of prominent fabrication and operating parameters. The findings reveal that increasing PAN concentration in dope and addition of citric acid were successful in tailoring membrane microstructure. Bacteria rejection in modified membranes improved by exhibiting high log removal values (LRV) ranging from 3.92 (99.87%) to 5.57 (99.99%) while permeate fluxes w
Some Staphylococcus aureus strains produce Panton-Valentine leukocidin (PVL), a bi-component pore-forming toxin, which causes leukocyte lysis and tissue necrosis. Currently, there is very limited information on the molecular epidemiology of PVL-encoding S. aureus strains in Iran. This study aimed to determine the molecular epidemiology and genetic background of PVL-positive S. aureus clinical strains isolated from Iranian patients. A total of 28 PVL-positive S. aureus strains were detected from 600 S. aureus isolates between February 2015 and March 2018 from different hospitals in Tehran, Iran. Antimicrobial susceptibility testing was performed according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. Molecular genot
Bioactive glasses (BGs) have attracted added attention in the structure of the scaffolds for bone repair applications. Different metal ions could be doped in BGs to induce specific biological responses. Among these ions, strontium (Sr) is considered as an effective and safe doping element with promising effects on bone formation and regeneration. In this experiment, we evaluated the antibacterial activities of the gelatin-BG (Gel-BG) and Gel-BG/Sr scaffolds in vitro. The osteogenic properties of the prepared scaffolds were also assessed in rabbit calvarial bone defects for 12 weeks. Both scaffolds showed in vivo bone formation during 12 weeks with the newly formed bone area in Gel-BG/Sr scaffold was higher than that in Gel-BG scaffolds afte
The aim of this study was to assess the modulatory effect of TcpA in the expression of CEACAM1 adhesin molecule and IL‐1, IL‐8 and TNFα pro‐inflammatiory cytokines in the co‐culture model of Caco‐2/PBMC that can mimic the intestinal milieu. The tcpA gene from V.cholerae ATCC14035 was cloned in pET‐28a and transformed into E.coli Bl‐21. The recombinant TcpA‐His6 protein was expressed and purified using the Ni‐column chromatography. The sequencing of transformed plasmid and Western blotting of purified protein confirmed the identity of rTcp. Cytotoxicity of different concentrations of recombinant protein for human colon carcinoma cell line (Caco‐2 cell) was assessed by MTT assay and showed viability of 92%, 82% and 70 %,
Nowadays, petroleum pollution is one of the most important environmental challenges in Iran. Some bacteria were isolated for hexadecane degrading from soil, sediment, and sludge; however, there is no report on its isolation from compost. This study was aimed to isolation, molecular identification of novel bacteria with high capability of hexadecane-degrading from compost using enrichments media. The isolated bacteria were identified by PCR with 16S rDNA method and were studied their ability for removing hexadecane in liquid and solid medium. According to results, the isolated bacteria were identified as O.oryzae and P.lautus. In liquid medium, hexadecane concentration decreased from 3000?1.4 to 366.96?0.9 mg/l (87.77?0.2%) by O.oryzae, whil
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